Yasuaki Hotta

Learn More
Microbial fuel cells (MFCs) are devices that exploit microorganisms to generate electric power from organic matter. Despite the development of efficient MFC reactors, the microbiology of electricity generation remains to be sufficiently understood. A laboratory-scale two-chamber microbial fuel cell (MFC) was inoculated with rice paddy field soil and fed(More)
A thermophilic syntrophic bacterium, Pelotomaculum thermopropionicum strain SI, was grown in a monoculture or coculture with a hydrogenotrophic methanogen, Methanothermobacter thermautotrophicus strain DeltaH. Microscopic observation revealed that cells of each organism were dispersed in a monoculture independent of the growth substrate. In a coculture,(More)
Two H-type microbial fuel cells were prepared. The anaerobic chambers were inoculated with rice paddy field soil and fed cellulose as an energy source. In one reactor, the anode and cathode were connected with a wire (closed circuit, CC), while they were not connected in the other reactor (open circuit, OC). The OC reactor actively produced methane. In the(More)
This study was conducted to examine the ultrastructures of eight recently improved light-cure restorative composite resins with scanning and transmission electron microscopes (SEM and TEM). Additionally, Vickers hardness, volume/weight fraction of filler, and chemical composition were analyzed. Composite resins selected for evaluation were Beautifil II,(More)
A simple model (termed the syntrophy model) for simulating the contribution of coaggregation to interspecies hydrogen fluxes between syntrophic bacteria and methanogenic archaea is described. We applied it to analyzing partially aggregated syntrophic cocultures with various substrates, revealing that large fractions of hydrogen molecules were fluxed in(More)
OBJECTIVE To examine the ultrastructure of six light-cure orthodontic adhesives with scanning electron microscope (SEM) and transmission electron microscope (TEM), microhardness tester, and energy dispersive X-ray microanalyzer (EDX). MATERIALS AND METHODS The orthodontic adhesives evaluated were Transbond XT, Light Bond, BeautyOrtho Bond, Kurasper F,(More)
BACKGROUND Sugar-protein glycocalyx coats healthy endothelium, but its ultrastructure is not well described. Our aim was to determine the three-dimensional ultrastructure of capillary endothelial glycocalyx in the heart, kidney, and liver, where capillaries are, respectively, continuous, fenestrated, and sinusoidal. METHODS Tissue samples were processed(More)
The toluene-degrading bacterium Acinetobacter sp. Tol 5 is highly adhesive to solid surfaces owing to two filamentous cell appendages, namely, anchors and peritrichate fibrils. When growing this bacterium in the presence of a carrier made of polyurethane foam, almost all the cells adhered to the surface of the carrier. In contrast, when Tol 5 cells were(More)
INTRODUCTION The purpose of this study was to observe, with a scanning electron microscope, the interface between enamel and orthodontic adhesive after focused ion-beam milling. In addition, enamel etched with phosphoric acid was compared with enamel conditioned with self-etching primer. METHODS Four freshly extracted human premolars were collected and(More)