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The upgrading of rapeseed cultivars has resulted in a substantial improvement in yield and quality in China over the past 30 years. With the selective pressure against fatty acid composition and oil content, high erucic acid- and low oil-content cultivars have been replaced by low erucic acid- and high oil-content cultivars. The high erucic acid cultivar(More)
Deinococcus geothermalis is a radioresistant and moderate thermophilic bacterium. Little was known about the membrane or membrane associated proteins of this bacterium. This study established the membrane subproteome profile of D. geothermalis, using 1-D PAGE and LC-MS/MS analysis following Triton X-114 detergent extraction. A total of 552 proteins from the(More)
A novel carotenoid 1,2-hydratase (CruF) responsible for the C-1',2' hydration of gamma-carotene was identified in the non-photosynthetic bacteria Deinococcus radiodurans R1 and Deinococcus geothermalis DSM 11300. Gene expression and disruption experiments demonstrated that dr0091 and dgeo2309 encode CruF in D. radiodurans and D. geothermalis, respectively.(More)
Fluorescence anisotropy (FA) is a reliable, sensitive, and robust assay approach for determination of many biological targets. However, it is generally not applicable for the assay of small molecules because their molecular masses are relatively too small to produce observable FA value changes. To address this issue, we report herein the development of a FA(More)
In this paper, we extend the implementable APG method to solve the matrix l2,1-norm minimization problem arising in multi-task feature learning. We investigate that the resulting inner subproblem has closed-form solution which can be easily determined by taking the problem’s favorable structures. Under suitable conditions, we can establish a comprehensive(More)
Up to now, the successful fabrication of efficient hot-spot substrates for surface-enhanced Raman scattering (SERS) remains an unsolved problem. To address this issue, we describe herein a universal aptamer-based SERS biodetection approach that uses a single-stranded DNA as a universal trigger (UT) to induce SERS-active hot-spot formation, allowing, in(More)
A highly sensitive and selective fluorescence aptamer biosensors for the determination of adenosine triphosphate (ATP) was developed. Binding of a target with splitting aptamers labeled with pyrene molecules form stable pyrene dimer in the γ-cyclodextrin (γ-CD) cavity, yielding a strong excimer emission. We have found that inclusion of pyrene dimer in(More)
In this communication, we molecularly engineered a light-switching excimer oligonucleotide-based probe for time-resolved fluorescent detection of Hg(2+). By combining a time-resolved fluorescence technique and magnetic nanoparticles, Hg(2+) spiked in human urine was detected.
In this paper, we propose SS-MAP, a scalable and Sybil-proof locator/ID mapping scheme for the future Internet. SS-MAP uses a near-optimal distributed hash table (DHT) to map identifiers onto locators in one hop with reasonable maintenance overhead, which are O(log n) hops in regular DHT-based mechanisms (n is the number of mapping servers). This is the(More)