Yao-Tsung Chang

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Optimization for power is always one of the most important design objectives in modern nanometer IC design. Recent studies have shown the effectiveness of applying multi-bit flip-flops to save the power consumption of the clock network. However, all the previous works applied multi-bit flip-flops at earlier design stages, which could be very difficult to(More)
In situ immobilization of heavy metals using reactive or stabilizing materials is a promising solution for soil remediation. Therefore, four agronomic and industrial by-products [wood biochar (WB), crushed oyster shell (OS), blast furnace slag (BFS), and fluidized-bed crystallized calcium (FBCC)] and CaCO3 were added to acidic soil (Cd = 8.71 mg kg(-1)) at(More)
Dendroaspin (Den) and rhodostomin (Rho) are snake venom proteins containing a PRGDMP motif. Although Den and Rho have different 3D structures, they are highly potent integrin inhibitors. To study their structure, function, and dynamics relationships, we expressed Den and Rho in Pichia pastoris. The recombinant Den and Rho inhibited platelet aggregation with(More)
Applying multi-bit flip-flops (MBFFs) for clock power reduction in modern nanometer ICs has been becoming a promising lower-power design technique. Many previous works tried to utilize as more MBFFs with larger bit numbers as possible to gain more clock power saving. However, an MBFF with a larger bit number may lead to serious crosstalk due to the close(More)
This study compared the extractability of Cd, Cu, Ni, Pb, and Zn by 8 extraction protocols for 22 representative rural soils in Taiwan and correlated the extractable amounts of the metals with their uptake by Chinese cabbage for developing an empirical model to predict metal phytoavailability based on soil properties. Chemical agents in these protocols(More)
Rhodostomin (Rho) is a medium disintegrin containing a 48PRGDMP motif. We here showed that Rho proteins with P48A, M52W, and P53N mutations can selectively inhibit integrin αIIbβ3. To study the roles of the RGD loop and C-terminal region in disintegrins, we expressed Rho 48PRGDMP and 48ARGDWN mutants in Pichia pastoris containing 65P, 65PR, 65PRYH,(More)
Rhodostomin (Rho) is an RGD protein that specifically inhibits integrins. We found that Rho mutants with the P48A mutation 4.4-11.5 times more actively inhibited integrin α5β1. Structural analysis showed that they have a similar 3D conformation for the RGD loop. Docking analysis also showed no difference between their interactions with integrin α5β1.(More)
Echistatin (Ech) is a potent inhibitor of integrins and was isolated from snake Echis carinatus. To facilitate the study on the molecular determinants of integrin-ligand interactions, we expressed recombinant Ech and its mutants in the Pichia pastoris (P. pastoris) expression system and purified them to homogeneity with the yields of 2-7 mg/L. Ech produced(More)
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