Yao Chou

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Exchange assays have often been used to quantitate steroid receptors when endogenous ligands are present; however, there are no reports of their successful application to activated glucocorticoid-Type II receptor complexes. In addition to investigating the reasons for this failure, the present study also examined the effects of progesterone on(More)
The aim of this study was to identify the antiviral mechanism of a novel compound, BPR3P0128. From a large-scale screening of a library of small compounds, BPR3P compounds were found to be potent inhibitors of influenza viral replication in Madin-Darby canine kidney (MDCK) cells. BPR3P0128 exhibited inhibitory activity against both influenza A and B(More)
In the present study we have examined the expression of mineralocorticoid Type I and glucocorticoid Type II receptors in astrocyte glia maintained in culture for different periods of time. Cytosolic mineralocorticoid Type I receptors were labeled with [3H]aldosterone (ALDO) in the presence of a 500-fold molar excess of the potent Type II receptor ligand RU(More)
Abstract The purpose of this study was to characterize and compare the properties of glucocorticoid Type II receptors in neuronal and astrocyte glial cultures prepared from rat brain. Type II receptors in cytosol prepared from cultured cells were labeled with [(3) H]dexamethasone (DEX) at 0 degrees C. The binding was saturable and specific, with a complete(More)
Keywords: The first-order derivative Computational precision Numerical differentiation 1-step-ahead Error analysis Optimal step length a b s t r a c t In order to achieve higher computational precision in approximating the first-order derivative of the target point, the 1-step-ahead numerical differentiation formulas are presented. These formulas greatly(More)
Membranes prepared from either neuronal or glial cultures contain alpha 2-adrenergic receptors as determined by the characteristics of [3H]yohimbine [( 3H]YOH) binding. The binding was rapid, reversible, saturable, dependent on the protein concentration used, and reached equilibrium by 5 min in membranes from both neuronal and glial cultures. Scatchard(More)
Hydrophobic interaction chromatography has been used to demonstrate an increase in the surface hydrophobicity of [3H]triamcinolone acetonide ([3H]TA)-labeled type II receptors in mouse brain cytosol following transformation of these receptor complexes to the activated DNA-binding form. After removing unbound [3H]TA and molybdate (which prevents activation)(More)
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