- Full text PDF available (1)
- This year (0)
- Last five years (0)
We describe physicochemical and enzymatic properties of 5' bridging phosphorothioester linkages at specific sites in DNA oligonucleotides. The susceptibility to hydrolysis at various pH values is examined and no measurable hydrolysis is observed at pH 5-9 after 4 days at 25 degrees C. The abilities of three 3'- and 5'-exonuclease enzymes to hydrolyze the… (More)
A new iodothymidine phosphoramidite enables the placement of a 5'-iodide into oligonucleotides; the iodide is stable to ammonia deprotection and allows nonenzymatic ligations of DNA.