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OBJECTIVE The aim of the study is to establish in vitro cell line with stable and effective 3Dpol gene expression, so as to study the biological function of foot-and-mouth disease virus (FMDV ) 3Dpol and foot-and-mouth disease (FMD) gene engineering vaccine. METHODS FMDV 3D gene was amplified from pMD18-T-3D and inserted into pGEM-Teasy vector. By(More)
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