Yanjun Zhang

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Escherichia coli DH5α grows poorly in a chemically defined medium, but its acetate-tolerant mutant, DA19, grows fast and produces less acetate. To investigate the differences in metabolism between the two strains, the samples of continuous cultures at steady state were analyzed by two-dimensional SDS-PAGE (2-D SDS-PAGE) and peptide mass fingerprinting(More)
Wnt signaling plays a key role in embryonic patterning and morphogenetic movements. The secreted Frizzled-related proteins (sFRPs) antagonize Wnt signaling, but their roles in development are poorly understood. To determine whether function of sFRPs is conserved between amphioxus and vertebrates, we characterized sFRP2-like function in the amphioxus,(More)
The interferon inducible p200 family proteins are expressed in a variety of cells and tissues. Intensive studies showed that they were involved in the regulation of cell proliferation and differentiation based on their ability to bind and modulate the activities of multiple transcription activators and inhibitors. Among p200 proteins, p203 has received the(More)
We report here the expression of AmphiMdp in embryos and larvae. Faint AmphiMdp transcripts were first detected in the mesendoderm at the mid-gastrula stage and later in the somites of the early neurula. Expression remained in somites throughout the neurula and early larval stages and then disappeared from the somites starting with the most anterior somite(More)
A full-length amphioxus γ-aminobutyric acid type-A receptor-associated protein-like 2 (GABARAPL2) cDNA was isolated. Its sequence and developmental expression are first described in this paper. The phylogenetic analysis shows that the amphioxus GABARAPL2 and GABARAPL2 in vertebrates are highly homologous. The results of in situ hybridization show that the(More)
Both mitosis and meiosis are driven by M-phase promoting factor (MPF), a complex with Cdc2 and Cyclin B. The concentration of Cdc2 remains relatively constant during the cell cycle, while the concentration of Cyclin B fluctuates periodically. Many studies have demonstrated high expression levels of Cdc2 and Cyclin B in the testis. In some gene knock-out(More)
OBJECTIVE To study the mechanism of the differences in cell growth and acetate production between Escherichia coli DH5alpha and its acetate-tolerant mutant DA19. METHODS Based on our previous analysis of the proteome and mass and energy balances for both strains, we first amplified the genes of atpIBEFHAGDC, purHD, and ndh, their regulation regions, as(More)
OBJECTIVE In order to further understand the difference in metabolic regulation between Escherichia coli DH5alpha and its acetate-tolerant mutant DA19, we analyzed the effect of supplementing adenine in defined media on metabolic fluxes in the two strains. METHODS E. coli DH5alpha and DA19 were continuously cultured in nitrogen-limited defined media(More)
The phytohormone salicylic acid (SA) plays essential roles in biotic and abiotic responses, plant development, and leaf senescence. 2,5-Dihydroxybenzoic acid (2,5-DHBA or gentisic acid) is one of the most commonly occurring aromatic acids in green plants and is assumed to be generated from SA, but the enzymes involved in its production remain obscure. DMR6(More)
Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating diseases of rice. However, the molecular mechanism underpinning the Xoo resistance of rice is still not fully understood. Here, we report that a class II small heat shock protein gene, OsHsp18.0, whose expression was differentially induced between a resistant and a(More)