Yang-Sun Chan

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Overexpression of the isopentenyltransferase gene (ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytokinin levels, leading to generation of shoots from transformed plant cells. When combined with a dexamethasone-inducible system for controlling expression, ipt expression can be used to select for transgenic regenerants without using an(More)
Cymbidium mosaic virus (CyMV) is the most prevalent virus infecting orchids. Here, we report the isolation of partial cDNA clones encoding the genomic RNA of CyMV. Like most of the polyadenylated monopartite positive-strand RNA viruses, the open reading frame (ORF) coding for the viral coat protein (CP) is located at the 3′ end. The ORF predicts a(More)
Figure 4. The tertiary structure of tRNA and helical regions that give high yield. A ribbon traces the RNA chain on wire-frame models of tRNA (derived from X-ray diffraction coordinates from the Protein Database). Positions of high yield in 3.5 M TMACl are marked red on the ribbon. Bases in single-stranded regions (yellow), which are stacked onto(More)
Figure 4. The tertiary structure of tRNA and helical regions that give high yield. A ribbon traces the RNA chain on wire-frame models of tRNA (derived from X-ray diffraction coordinates from the Protein Database). Positions of high yield in 3.5 M TMACl are marked red on the ribbon. Bases in single-stranded regions (yellow), which are stacked onto(More)
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