Yan-ying Fan

Learn More
Human natural killer (NK) cells are one major component of lymphocytes that mediate early protection against viruses and tumor cells, and play an important role in immune regulatory functions. In this study, we demonstrated that human NK cells could be divided into four subsets, CD56hi CD16(-), CD56lo CD16(-), CD56+CD16+ and CD56(-)CD16+, based on the(More)
AIM To investigate the role of cytokines (IL-23, IL-2 and IL-15) in the production and regulation of IL-17 and IFN-gamma via the induction of normal human peripheral blood mononuclear cells (PBMCs) and CD4(+) T cells. METHODS PBMCs or CD4(+) T cells from normal human blood were cultured with IL-23, IL-2 or IL-15 in the presence or absence of IL-23. The(More)
AIM To study the effect and mechanism of BCG on human nature killer cells. METHODS PBMC or purified NK cells were isolated from normal human peripheral blood with negative anti-Mycobacterium tuberculosis antibody and cultured with BCG, IL-12, BCG plus IL-12 and BCG plus anti-IL-12R beta 1 mAb (2B10), respectively. The levels of IFN-gamma and IL-12p40 in(More)
Natural killer cells (NK) from umbilical cord blood (CB) play an important role in allogeneic stem cell transplantation and defending infections of newborn. Based on the surface expression of CD56 and CD16 or inhibitory and activatory receptors, NK cells could be subdivided into various subsets with distinct functions. To investigate the biological(More)
BACKGROUND & OBJECTIVE Currently, 5-fluorouracil (5-FU) is still one of the widely applied chemotherapeutic agents for tumors. Interleukin-12 (IL-12) can promote the differentiation of Th1 cells and induce the production of interferon-gamma (IFN-gamma) by CD8+ T cells. This study was to investigate the suppression mechanism of 5-FU on the immune response(More)
SARS-CoV infection of human results in antigen-specific cellular and humoral immune responses. However, it is critical to determine whether SARS-CoV-specific memory T cells can persist for long periods of time. In this study, we analyzed the cellular immune response from 21 SARS-recovered individuals who had been diagnosed with SARS in 2003 by using ELISA,(More)
AIM To investigate the effect of the TLR ligand (R-848) and IL-12 on the production of IFN-gamma by human NK cell subsets. METHODS PBMC or purified NK cells were isolated from normal human peripheral blood and cultured with R-848, IL-12 , R-848+ IL-12. The level of IFN-gamma in the culture supernatants was measured by ELISA. The cell subsets which(More)
  • 1