Yan Bo Sun

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1. The fluorescent dye fluo-3, in its permeant acetoxymethyl form, was used to monitor calcium transients during twitch and tetanus of single fibres isolated from the anterior tibialis muscle of Rana temporaria (2-5 degrees C). 2. Fluo-3 was loaded into the muscle fibre by diffusion. Under the experimental conditions used, approximately 45% of maximal(More)
The calcium-sensitive fluorescent indicator fluo-3 was used to monitor the intracellular free calcium concentration ([Ca2+]i) during isometric twitches in twenty-nine single muscle fibres from the anterior tibialis muscle of Rana temporaria (sarcomere length, 2.2 microns; 2-4 degrees C). The transient change in [Ca2+]i in response to a single stimulus was(More)
The effects of 2,3-butanedione monoxime (BDM) on the force-velocity relation were studied in single fibres from the anterior tibialis muscle of Rana temporaria (2.2 microns sarcomere length, temperature 1.9-2.4 degrees C). BDM (1.0 and 1.8 mM) suppressed the maximum tetanic force (P0) and the maximum speed of shortening (Vmax), and increased the main(More)
The effects of 2,3-butanedione monoxime (BDM) on intracellular Ca2+ transient and cross-bridge function were studied in frog single fibres from the anterior tibialis muscle of Rana temporaria (sarcomere length, 2.2 microm; temperature, 2-4 degrees C). The fluorescent dye fluo-3 was used to monitor the intracellular free calcium concentration ([Ca2+]i)(More)
The effects of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) on force and intracellular Ca2+ transient were studied during isometric twitches and tetanuses in single frog muscle fibers. BAPTA was added to the bathing solution in its permeant AM form (50 and 100 microM). There was no clear correlation between the changes in force and the(More)
Single fibres from the anterior tibialis muscle of Rana temporaria (temperature, 2-3 degrees C) were moderately fatigued at a prestretched sarcomere length (approximately 3.6 microns) by reducing the intervals between 2 s tetani from 240 to 20 s. Changes in length of marked segments along the muscle fibre were monitored during fixed-end tetani using a(More)
The force-velocity relation has been studied during calcium-induced contracture of chemically skinned fibres from the semitendinosus muscle of Rana temporaria with special interest focused on the high-load region. The force-velocity curve was hyperbolic at low and intermediate loads but departed below the hyperbola as the load exceeded about 80% of the(More)
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