Yakup Kolcuoğlu

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The D-glucose/D-xylose isomerase was purified from a thermophilic bacterium, Geobacillus thermodenitrificans TH2, by precipitating with heat shock and using Q-Sepharose ion exchange column chromatography, and then characterized. The purified enzyme had a single band having molecular weight of 49 kDa on SDS-PAGE. In the presence of D-glucose as a substrate,(More)
Results: The recombinant enzyme had 1326 bp and optimum pH and temperature of the XyI were found to be 6.5 and 80°C, respectively. At 4.0-9.0 pH range and 4°C, after 15 days incubation, the enzyme was quite stable. The enzyme retained nearly 80% of its original activity after 3 hours incubation at 60°C and 70°C. In the presence of glucose as substrate, Km(More)
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