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The gene encoding for a pumpkin (Curcubita maxima) trypsin inhibitor CMTI-V was synthesized chemically. The synthetic gene was prepared from four overlapping oligonucleotides by overlapping extension. The synthetic gene was amplified by polymerase chain reaction, cloned into a T7 expression vector and expressed in Escherichia coli as a fusion protein. The(More)
The help information provided by search engines can facilitate its user's information seeking process or hinder it. This study analyzed 20 search engines on how their help systems are placed and organized. Help systems are separated into pre-search help system, and post-search help system, and six aspects of help systems were investigated, including(More)
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