YASUSHI HAMAGUCHI

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Rabbit morulae were exposed to a vitrification solution-modified PBS [PB1] medium containing 40% ethylene glycol + 18% Ficoll + 0.3 M sucrose (EFS) for 2, 5, or 10 min at 20 degrees C and were vitrified in liquid nitrogen. When morulae were rapidly warmed, 96% had an intact zona pellucida. When embryos were cultured after removal of the mucin coat, high(More)
Dietary cholesterol suppressed adjuvant arthritis, a chronic inflammatory disease, in rats, but did not significantly affect carrageenin edema, an acute inflammation. When rats were fed a high-cholesterol diet beginning 10 days before injection of adjuvant, the development of the adjuvant-induced arthritis was greatly suppressed. Cholesterol feeding(More)
This study offers findings which should aid in the development of a convenient animal model of atherosclerosis. Inbred Fisher strain rats were fed an atherogenic diet containing 1.5% cholesterol and 0.5% cholic acid and given a single subcutaneous injection of adjuvant (Mycobacterium butyricum) into the base of the tail. The animals were maintained for 8(More)
Effects of SE-1520 were compared with those of thiazide diuretics, hydrochlorothiazide (HCT) and trichlormethiazide (TCM) in rats and dogs. SE-1520 in an oral dose of 0.1 approximately 10.0 mg/kg resulted in a strong and dose-dependent increase in urine volume as well as in the urinary excretion of Na+ and Cl-, while that of K+ significantly increased with(More)
Effect of Colcemid treatment of myeloma (X63-Ag8-6.5.3.) prior to fusion with mouse spleen cell was studied in terms of hybridoma formation. Spleen cells from BALB/c mice immunized with various soluble antigens were fused with the myeloma cells by using polyethylene glycol solution. Colcemid treatment of myeloma cells prior to fusion increased the average(More)
Mast cell precursors are derived from multipotential hematopoietic stem cells, migrate in the blood stream, and enter the tissue where they differentiate into morphologically identifiable mast cells (reviewed in reference 1) . The differentiated mast cells may be classified into at least two phenotypically distinct populations; connective tissue-type mast(More)
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