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A Novel Alkaliphilic Bacillus Esterase Belongs to the 13th Bacterial Lipolytic Enzyme Family
Background Microbial derived lipolytic hydrolysts are an important class of biocatalysts because of their huge abundance and ability to display bioactivities under extreme conditions. In spite of… Expand
Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4
- Y. Zheng, Y. Xue, Y. Zhang, C. Zhou, U. Schwaneberg, Y. Ma
- Biology, Medicine
- Applied Microbiology and Biotechnology
- 13 February 2010
A thermostable glucoamylase (TtcGA) from Thermoanaerobacter tengcongensis MB4 was successfully expressed in Escherichia coli. The full-length gene (2112 bp) encodes a 703-amino acid polypeptide… Expand
Structural insight into catalytic mechanism of PET hydrolase
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures… Expand
Functional and structural studies of pullulanase from Anoxybacillus sp. LM18‐11
Pullulanase is a debranching enzyme that specifically hydrolyzes the α‐1,6 glycosidic linkage of α‐glucans, and has wide industrial applications. Here, we report structural and functional studies of… Expand
The substrate/product-binding modes of a novel GH120 β-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485
- Chun-Hsiang Huang, Y. Sun, +7 authors R. Guo
- Chemistry, Medicine
- The Biochemical journal
- 20 September 2012
Xylan-1,4-β-xylosidase (β-xylosidase) hydrolyses xylo-oligomers at their non-reducing ends into individual xylose units. Recently, XylC, a β-xylosidase from Thermoanaerobacterium saccharolyticum… Expand
Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.
The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). Unlike other Rex proteins that regulate aerobic respiration, RSP controls ethanol fermentation in the obligate… Expand
Polysaccharide-based nanocomposites and their applications.
Polysaccharide nanocomposites have become increasingly important materials over the past decade. Polysaccharides offer a green alternative to synthetic polymers in the preparation of soft… Expand
Biomimetic composite scaffold of hydroxyapatite/gelatin-chitosan core-shell nanofibers for bone tissue engineering.
- Peng Chen, Leyun Liu, Jiaqi Pan, J. Mei, C. Li, Y. Zheng
- Materials Science, Medicine
- Materials science & engineering. C, Materials for…
- 1 April 2019
Good biocompatibility and osteogenesis of three-dimensional porous scaffolds are critical for bone tissue engineering. In this work, biomimetic hydroxyapatite/gelatin-chitosan core-shell nanofibers… Expand
Structural perspectives of an engineered β-1,4-xylanase with enhanced thermostability.
The glycoside hydrolase 10 (GH10) xylanase from Streptomyces sp. 9 (XynAS9) can operate in a broad range of pH and temperature, and thus is a potential candidate for commercial applications.… Expand
Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum
We report the first X-ray crystal structure of ent-kaur-16-ene synthase from Bradyrhizobium japonicum, together with the results of a site-directed mutagenesis investigation into catalytic activity.… Expand