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Biosynthesis of the allylmalonyl-CoA extender unit for the FK506 polyketide synthase proceeds through a dedicated polyketide synthase and facilitates the mutasynthesis of analogues.
TLDR
This unique feature of FK506 biosynthesis, in which a dedicated PKS provides an atypical extender unit for the main modular PKS, illuminates a new strategy for the combinatorial biosynthesis of designer macrolide scaffolds as well as FK505 analogues.
The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)
TLDR
Nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete, are integrated and it is revealed that the translation efficiency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase.
Enhanced FK506 production in Streptomyces clavuligerus CKD1119 by engineering the supply of methylmalonyl-CoA precursor
TLDR
Results show that the methylmalonyl-CoA supply is a limiting factor for FK506 biosynthesis and that among the three pathways analyzed, the MCM pathway is the most effective precursor supply pathway supporting the highest titer of Fk506 in S. clavuligerus CKD1119.
Roles of fkbN in Positive Regulation and tcs7 in Negative Regulation of FK506 Biosynthesis in Streptomyces sp. Strain KCTC 11604BP
TLDR
Semiquantitative reverse transcription-PCR analyses of the transcription levels of the FK506 biosynthetic genes in the wild-type and mutant strains proved that most of the fkbN encodes a positive regulatory protein essential for Fk506/FK520 biosynthesis and that the gene product of tcs7 negatively regulates their biosynthesis, demonstrating the potential of exploited for strain improvement.
Immobilization of Horseradish Peroxidase on Multi-Wall Carbon Nanotubes and its Electrochemical Properties
Horseradish peroxidase (HRP) was immobilized on carboxylated multi-wall carbon nanotubes in the presence of a coupling reagent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. The immobilized HRP
A Review of the Microbial Production of Bioactive Natural Products and Biologics
TLDR
The structures and diverse biological activities of natural products and recombinant proteins that have been exploited as valuable molecules in medicine, agriculture and insect control are introduced and suggested to inspire the development of new therapeutic agents in academia and industry.
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
TLDR
It is discovered that the kanamycin biosynthetic pathway contains an early branch point, governed by the substrate promiscuity of a glycosyltransferase, that leads to the formation of two parallel pathways in which early intermediates are further modified.
Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae.
TLDR
This work expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols.
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