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Orientation of Asymmetric Stem Cell Division by the APC Tumor Suppressor and Centrosome
It is shown that dividing Drosophila male germline stem cells use intracellular mechanisms involving centrosome function and cortically localized Adenomatous Polyposis Coli tumor suppressor protein to orient mitotic spindles perpendicular to the niche, ensuring a reliably asymmetric outcome.
Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover
The results show that deadenylation is the key first step that triggers decay of both wild-type stable and nonsense codon–containing unstable β-globin mRNAs in mouse NIH3T3 fibroblasts.
Dynamics of centromeres during metaphase-anaphase transition in fission yeast: Dis1 is implicated in force balance in metaphase bipolar spindle.
The Centromere showed dynamic movements throughout phase 2 as it moved back and forth and was transiently split in two before its separation, suggesting that the centromere was positioned in a bioriented manner toward the poles at metaphase.
Centrosome misorientation reduces stem cell division during ageing
It is proposed that cell cycle arrest associated with centrosome misorientation functions as a mechanism to ensure asymmetric stem cell division, and that the inability of stem cells to maintain correct orientation during ageing contributes to the decline in spermatogenesis.
Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
Fission yeast temperature‐sensitive mutants cut3‐477 and cut14‐208 fail to condense chromosomes but small portions of the chromosomes can separate along the spindle during mitosis, producing…
Asymmetric Inheritance of Mother Versus Daughter Centrosome in Stem Cell Division
It is shown that developmentally programmed asymmetric behavior and inheritance of mother and daughter centrosomes underlies the stereotyped spindle orientation and asymmetric outcome of stem cell divisions in the Drosophila male germ line.
20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway
It is shown that Cut4 (ref. 6) is an essential component of the cyclosome in fission yeast and suppression correlates with the amount of 20S complex, indicating that cyclosomes formation and activation is inhibited by the cAMP/PKA pathway.
Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy
This work identifies loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders.
Human TOB, an Antiproliferative Transcription Factor, Is a Poly(A)-Binding Protein-Dependent Positive Regulator of Cytoplasmic mRNA Deadenylation
Results from glutathione S-transferase pull-down and coimmunoprecipitation experiments indicate that TOB can simultaneously interact with the poly(A) nuclease complex CCR4-CAF1 and the cytoplasmicpoly(A)-binding protein, PABPC1.
Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells
Surprisingly perhaps, given the role of Mre11 in yeast NHEJ, disruption of N HEJ by deletion of KU70 greatly exacerbates the effects of MRE11 deficiency, revealing a significant Mre 11‐independent component of metazoan NHEj.