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Gene expression in tobacco low-nicotine mutants.
Results suggest that Nic1 and Nic2 are regulatory genes for nicotine biosynthesis, and exogenous supply of auxin down-regulated both genes in cultured tobacco roots. Expand
Directed disruption of the tobacco ndhB gene impairs cyclic electron flow around photosystem I.
Analysis of the transient increase in chlorophyll fluorescence after actinic light illumination and the redox kinetics of P700 suggest that the cyclic electron flow around PS I is impaired in the ndhB-deficient transformants, suggesting that the cycling of electrons aroundPS I mediated by ndh gene products is dispensable in tobacco under mild environmental conditions. Expand
Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the alpha-Proteobacteria.
Eight Gram-negative, aerobic, rod-shaped and peritrichously flagellated strains were isolated from flowers of the orchid tree and of plumbago and from fermented glutinous rice, all collected in Indonesia, and studies of DNA relatedness showed that they constitute a single species. Expand
The phylogeny of acetic acid bacteria based on the partial sequences of 16S ribosomal RNA: the elevation of the subgenus Gluconoacetobacter to the generic level.
The data obtained here indicated that the members of the subgenus Gluconoacetobacter of the genus Acetobacter can be distinguished at the generic level. Expand
A complex role for the gamma-butyrolactone SCB1 in regulating antibiotic production in Streptomyces coelicolor A3(2).
Many streptomycetes produce extracellular gamma-butyrolactones. In several cases, these have been shown to act as signals for the onset of antibiotic production. Synthesis of these molecules appearsExpand
Molecular cloning of hyoscyamine 6 beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger.
With computer-aided comparison to other proteins, it is found that the hydroxylase is homologous to two synthases involved in the biosynthesis of beta-lactam antibiotics in some microorganisms and the gene products of tomato pTOM13 cDNA and maize A2 locus which had been proposed to catalyze oxidative reactions in the BIOSynthesis of ethylene and anthocyan, respectively. Expand
Metabolic engineering of plant alkaloid biosynthesis.
Plant alkaloids, one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the biosynthetic pathways for scopolamine, nicotine, and berberineExpand
Jasmonate induction of putrescine N-methyltransferase genes in the root of Nicotiana sylvestris.
Qualitatively similar patterns of expression for the NsPMT genes are achieved with only 0.25 kb of their conserved 5'-flanking regions, which contained no known jasmonate-responsive elements. Expand
Expression of Atropa belladonna putrescine N-methyltransferase gene in root pericycle.
Histochemical analysis showed that GUS is expressed specifically in root pericycle cells and that the 0.3-kb 5'-upstream region was sufficient for pericycle-specific expression, and the regulation of tropane alkaloid biosynthesis is discussed and compared with that of nicotine biosynthesis. Expand
Putrescine N-Methyltransferase in Cultured Roots of Hyoscyamus albus: n-Butylamine as a Potent Inhibitor of the Transferase both in Vitro and in Vivo.
PMT was partially purified and characterized from cultured roots of Hyoscyamus albus that contain hyoscyamine as the main alkaloid and Monoamines were good competitive inhibitors of PMT, among which n-butylamine, cyclohexylamines, and exo-2-aminonorbornane were most inhibitory. Expand