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Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi.
Chitinase, beta-1,3-glucanase, and protease activities were formed when Trichoderma harzianum mycelia, grown on glucose as the sole carbon source, were transferred to fresh medium containing cellExpand
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Nuclear domains of the RNA subunit of RNase P.
The ribonucleoprotein enzyme RNase P catalyzes the 5' processing of pre-transfer RNA, and has also recently been implicated in pre-ribosomal RNA processing. In the present investigation, in situExpand
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Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells
The dynamic intra-nuclear localization of MRP RNA, the RNA component of the ribonucleoprotein enzyme RNase MRP, was examined in living cells by the method of fluorescent RNA cytochemistry (Wang, J.,Expand
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Mobility of cytoplasmic and membrane-associated actin in living cells.
We have combined fluorescent analogue cytochemistry with fluorescence photobleaching recovery to measure the mobility of fluorescently labeled actin and other labeled test proteins microinjected intoExpand
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Localization of pre-messenger RNA at discrete nuclear sites.
We have studied the nuclear localization of rhodamine-labeled pre-mRNA after microinjection into nuclei of cultured rat kidney epithelial cells. Intranuclear localization of the injected RNA wasExpand
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CP1 domain in Escherichia coli leucyl-tRNA synthetase is crucial for its editing function.
The amino acid discrimination by aminoacyl-tRNA synthetase is achieved through two sifting steps; amino acids larger than the cognate substrate are rejected by a "coarse sieve", while the reactionExpand
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Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin.
  • Y. Wang, D. L. Taylor
  • Chemistry, Medicine
  • The journal of histochemistry and cytochemistry…
  • 1 November 1980
The new technique of molecular cytochemitry (Taylor DL, Wang YL (1978): Proc Natl Acad Sci USA 75:857) requires the use of functional fluorescent analogs of cellular components with optimalExpand
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A modified procedure for fast purification of T7 RNA polymerase.
The in vitro T7 transcription system allows one to synthesize biochemical amounts of RNA molecules functionally equivalent or similar to those transcripts normally existing at extremely low levels inExpand
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Fluorescent analog cytochemistry of contractile proteins.
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Laminar flow in the inlet section of parallel plates
Velocity distributions, pressure gradients, and overall pressure drops are presented. A definite concavity was found in the velocity profile near the entrance for case I. In case II, which is moreExpand
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