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Minimum Information about a Biosynthetic Gene cluster.
This work proposes the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard, to facilitate consistent and systematic deposition and retrieval of data on biosynthetic gene clusters. Expand
Establishing a New Methodology for Genome Mining and Biosynthesis of Polyketides and Peptides through Yeast Molecular Genetics
This work has developed a streamlined method for isolating biosynthetic genes from fungal sources and producing bioactive molecules in an engineered Saccharomyces cerevisiae host strain using overlap extension PCR and yeast homologous recombination. Expand
Targeted disruption of transcriptional regulators in Chaetomium globosum activates biosynthetic pathways and reveals transcriptional regulator-like behavior of aureonitol.
Successful engineering of Chaetomium globosum is reported, a known producer of various valuable natural products, that allows its genetic manipulation via targeted homologous recombination to facilitate exploration of the untapped potential of fungal biosynthetic capability and identification of various unique biological functions that those secondary metabolites possess. Expand
Elucidation of pseurotin biosynthetic pathway points to trans-acting C-methyltransferase: generation of chemical diversity.
The study revealed the combinatorial nature of the biosynthesis of the pseurotin family of compounds and the intermediates and reported the first identification of an epoxidase C-methyltransferase bifunctional fusion protein PsoF which appears to methylate the nascent polyketide backbone carbon atom in trans. Expand
Elucidation of Pyranonigrin Biosynthetic Pathway Reveals a Mode of Tetramic Acid, Fused γ-Pyrone, and exo-Methylene Formation.
Successful activation of the pyranonigrin biosynthetic gene cluster and gene knockout in Aspergillus niger plus in vivo and in vitro assays led to isolation of six new products, including a spiroExpand
Tumescenamide C, an antimicrobial cyclic lipodepsipeptide from Streptomyces sp.
Tumescenamide C, a new cyclic lipodepsipeptide, was isolated from a culture broth of an actinomycete Streptomyces sp. KUSC_F05. Tumescenamide C was a congener of tumescenamides A and B, representingExpand
Echinomycin biosynthesis.
The echinomycin biosynthetic pathway was successfully reconstituted in Escherichia coli and an unrelated disulfide-forming enzyme GliT for gliotoxin biosynthesis was also able to catalyze the same reaction, providing another example of broad substrate specificity in secondary metabolite biosynthesis enzymes. Expand
Association between dietary intake and the prevalence of tumourigenic bacteria in the gut microbiota of middle-aged Japanese adults
The relationship between dietary intake and the prevalence of pks+ E. coli was examined in 223 healthy Japanese individuals to provide insight into potential dietary interventions for the prevention of CRC. Expand
Relationship between Habs serotypes and 2-amino sugar composition of Pseudomonas aeruginosa
Thirteen Habs-serotype strains could be classified into 11 groups depending on the characteristic patterns of 2-amino sugar composition; strains of serotypes 2, 5, and 7 had the same pattern, andExpand
Distinct mechanisms for spiro-carbon formation reveal biosynthetic pathway crosstalk.
The results highlight the versatile role of oxygenating enzymes in the biosynthesis of structurally complex natural products and indicate that cross-talk of different biosynthetic pathways allows product diversification in natural product biosynthesis. Expand