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Phylogenetic relationships of Eurasian pines (Pinus, Pinaceae) based on chloroplast rbcL, MATK, RPL20-RPS18 spacer, and TRNV intron sequences.
Phylogenetic analyses based on these four sequences gave consistent results and strongly supported the monophyly hypothesis for the genus Pinus and its two recognized subgenera. Expand
DNA variation in a conifer, Cryptomeria japonica (Cupressaceae sensu lato).
The frequency spectrum of the nucleotide polymorphism revealed excesses of intermediate-frequency variants, which suggests that the population was not panmictic and a constant size in the past, and suggested possibilities of natural selection acting at some of the loci. Expand
Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica)
Because they employ longer primers than do RAPDs, have a high degree of polymorphism, conform well to Mendelian expectations, and do not require use of acrylamide gels for analysis, ISSRs may be useful markers for PCR-based genome maps and population studies of conifers. Expand
Estimation of gene flow in the tropical‐rainforest tree Neobalanocarpus heimii (Dipterocarpaceae), inferred from paternity analysis
It is suggested that long‐distance gene flow and seed migration are responsible for the poorly developed genetic structure of this species. Expand
Microsatellite markers reveal high allelic variation in natural populations of Cryptomeria japonica near refugial areas of the last glacial period
Using 11 microsatellite markers, the allelic variation and genetic structure of Cryptomeria japonica is investigated, across most of its natural distribution, with a trend towards high allelic diversity in five populations, which are very close to, or in, refugial areas of the last glacial period as defined by Tsukada based on pollen analysis data and current climatic divisions. Expand
Intraspecific variation and phylogeographic patterns of Fagus crenata (Fagaceae) mitochondrial DNA.
The mtDNA variation displayed strong geographic structure, which may reflect the species' distribution in the last glacial maximum and subsequent colonization, and probably also reflects intraspecific phylogeography of the species. Expand
Development and polymorphism of simple sequence repeat DNA markers for Shorea curtisii and other Dipterocarpaceae species
Nine simple sequence repeat (SSR) markers were developed from Shorea curtisii using two different methods and revealed that all SSR loci were polymorphic, which means that the SSR primers for S.Curtisii can be applied to almost all species of Dipterocarpaceae. Expand
A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica
An open scheme for analyzing microsatellites or simple sequence repeats in expressed sequence tags (ESTs) and developing EST-SSR markers from reads collected by Sanger sequencing and pyrosequencing of sugi (Cryptomeria japonica), which results will be useful in genomic analyses of conifers and other non-model species. Expand
Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes
Dipterocarpaceae is the dominant family of Southeast Asia's climax tropical rain forest region, and it contains the region's most important commercial timber species. A molecular phylogeny of theExpand
Genetic diversity in Fagus crenata (Japanese beech): influence of the distributional shift during the late-Quaternary
Genetic diversity at 11 loci encoding nine enzymes was studied in 23 populations of Japanese beech Fagus crenata Blume distributed throughout the range of the species, and principal components analysis revealed that the populations tended to cluster according to their geographical locations. Expand