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Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
It is demonstrated that guaiacyl and syringyl lignin subunits are largely dispensable for normal growth and development, and it is suggested that the transcription machinery and signalling pathways responding to cell wall defects may be important targets to include in efforts to reduce biomass recalcitrance. Expand
Laccases Direct Lignification in the Discrete Secondary Cell Wall Domains of Protoxylem1[W][OPEN]
The data support a model of protoxylem TE lignification in which monolignols are highly mobile once exported to the cell wall, and in which precise targeting of laccases to secondary cell wall domains directs lignin deposition. Expand
A polymer of caffeyl alcohol in plant seeds
Data support the contention that the C-lignin polymer is produced in vivo via combinatorial oxidative radical coupling that is under simple chemical control, a mechanism analogous to that theorized for classical lignin biosynthesis. Expand
Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure
Results suggest that hydroxycinnamaldehyde content is a more important determinant of digestibility than lignin content in CAD-deficient plants. Expand
Syringyl lignin production in conifers: Proof of concept in a Pine tracheary element system
This study shows that metabolic engineering can be used to imbue pine tracheary elements with an ability to synthesize sinapyl alcohol, a lignin monomer not normally used for lignification in conifers such as pine, suggesting that it might be possible to retain the outstanding fiber properties of softwoods while imbuing them with the lign in characteristics of hardwoods that are more favorable for industrial processing. Expand
Loss of function of cinnamyl alcohol dehydrogenase 1 leads to unconventional lignin and a temperature-sensitive growth defect in Medicago truncatula
Glycome profiling revealed an increased extractability of some xylan and pectin epitopes from the cell walls of the cad1-1 mutant but decreased Extractability of others, suggesting that aldehyde-dominant lignin significantly alters cell wall structure. Expand
Suppression of CCR impacts metabolite profile and cell wall composition in Pinus radiata tracheary elements
Interestingly, CCR suppression substantially promoted pyrolytic breakdown of cell wall polysaccharides, a phenotype most likely caused by the incorporation of acidic compounds into the cell wall matrix in CCR-RNAi lines. Expand
Coexistence but Independent Biosynthesis of Catechyl and Guaiacyl/Syringyl Lignin Polymers in Seed Coats[W][OPEN]
This study provides evidence that several dicot plants (Euphorbiaceae and Cleomaceae) produce two distinctively different types of lignin polymers (i.e., catechyl and guaiacyl/syringyl lignins) in aExpand
CCoAOMT suppression modifies lignin composition in Pinus radiata.
A cDNA clone encoding the lignin-related enzyme caffeoyl’CoA 3-O-methyltransferase (CCoAOMT) was isolated from a Pinus radiata cDNA library derived from differentiating xylem, and 2D-NMR spectra suggested that phenylpropanoids with an ortho-diphenyl structure such as caffeyl alcohol are involved in lign in polymerization. Expand
Novel seed coat lignins in the Cactaceae: structure, distribution and implications for the evolution of lignin diversity.
The lignin types in the seed coats of 130 different cactus species are determined and a homopolymer formed by endwise β-O-4-coupling of caffeyl alcohol monomers onto the growing polymer resulting in benzodioxane units is shown. Expand