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Isolation and Characterization of an Alcohol Dehydrogenase Gene from the Octylphenol Polyethoxylate Degrader Pseudomonas putida S-5
TLDR
Transcription analysis using RT-PCR showed that adh1 is cotranscribed with the putative acyl-CoA synthetase and esterase genes during growth on OPEOn, and showed significant sequence similarity to bacterial alcohol dehydrogenases.
Molecular Characterization of a Lipoxygenase from the Basidiomycete Mushroom Pleurotus ostreatus
TLDR
Southern blot analysis indicated that PoLOX1 is a member of a small gene family comprising highly similar genes, and northern blot analysis revealed that it is transcribed more abundantly in the stipes of the fruit bodies than in the caps.
Heterogeneous expression and emulsifying activity of class I hydrophobin from Pholiota nameko
TLDR
This work has attempted to express class I hydrophobin PNH1 from Pholiota nameko fused with glutathione S-transferase (GST) in Escherichia coli, and revealed that the purified GST–PNH1 had almost the same emulsifying activity as P NH1.
Three genes specifically expressed during phosphate deficiency in Pholiota nameko strain N2 encode hydrophobins
TLDR
Phylogenetic analysis showed that PNH1, PNH2 and PNH3 were phylogenetically similar and significantly related to the hydrophobin POH1 specifically expressed in fruiting bodies of Pleurotus ostreatus.
Gene Expression during Pi Deficiency in Pholiota nameko: Accumulation of mRNAs for Two Transporters
TLDR
The effects of Pi deficiency on gene expression in Pholiota nameko showed that these pdi genes were expressed in various patterns during Pi deficiency, and deduced amino acid sequences indicated that they have structural similarities to Pi and metabolite transporters.
Structure and expression of a phosphate deficiency-inducible ribonuclease gene in Pholiota nameko
TLDR
Genomic DNA harboring the pdi370 gene was isolated from a genomic library of P. nameko and showed high similarity to fungal ribonucleases (RNases) and contained two signature sequences conserved in T2 family RNases: CAS1 and CAS2.
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