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New insights on trehalose: a multifunctional molecule.
This sugar is present in a wide variety of organisms, including bacteria, yeast, fungi, insects, invertebrates, and lower and higher plants, where it may serve as a source of energy and carbon and as a signaling molecule to direct or control certain metabolic pathways or even to affect growth. Expand
Castanospermine inhibits the processing of the oligosaccharide portion of the influenza viral hemagglutinin.
- Y. T. Pan, H. Hori, R. Saul, B. Sanford, R. Molyneux, A. Elbein
- Chemistry, Medicine
- 2 August 1983
Castanospermine proved to be a potent inhibitor of glycoprotein processing by virtue of the fact that it inhibits glucosidase I, and influenza virus produced in the presence of this alkaloid were fully infective and apparently produced in similar amounts to that of control cells, as determined by plaque counts. Expand
Trehalose synthase converts glycogen to trehalose
- Y. T. Pan, J. Carroll, N. Asano, I. Pastuszak, V. K. Edavana, A. Elbein
- Biology, Medicine
- The FEBS journal
- 1 July 2008
It is shown that TreS from Mycobacterium’smegmatis, as well as recombinant TreS produced in Escherichia’coli, has amylase activity in addition to the maltose interconverting activity (referred to as MTase), and that trehalose and TreS are both involved in the production of glycogen, and that the metabolism of tre Halose and glycogen is interconnected. Expand
Trehalose-phosphate synthase of Mycobacterium tuberculosis. Cloning, expression and properties of the recombinant enzyme.
These studies prove conclusively that the mycobacterial TPS is indeed responsible for catalyzing the synthesis of trehalose-P from any of the nucleoside diphosphate glucose derivatives. Expand
Trehalose-P synthase of mycobacteria: its substrate specificity is affected by polyanions.
The purified mycobacterial enzyme catalyzed the synthesis of trehalose-P from glucose-6-P and a variety of nucleoside diphosphate glucose derivatives, depending on whether a polyanion was absent or present, and UDP-glucose and GDP- glucose were the best glucosyl donors. Expand
Biological activities of the nortropane alkaloid, calystegine B2, and analogs: structure-function relationships.
- A. Goldmann, B. Message, +5 authors A. Elbein
- Biology, Medicine
- Journal of natural products
- 23 December 1996
Assays using synthetic (+)- and (-)-enantiomers of calystegine B2 established that catabolism by Rhizobium meliloti, glycosidase inhibition, and allelopathic activities were uniquely associated with the natural, (+)- enantiomer, and this N-methyl analog could serve to construct a cellular or animal model for Fabry's disease. Expand
Inhibition of glycoprotein processing by L-fructose and L-xylulose.
- S. Muniruzzaman, Y. T. Pan, Y. Zeng, B. Atkins, K. Izumori, A. Elbein
- Chemistry, Medicine
- 1 December 1996
When influenza virus-infected MDCK cells were raised in the presence of L-xylulose, there was a dose-dependent inhibition in the formation of complex types of oligosaccharides on the viral glycoproteins consistent with the inhibition of the processing glucosidase I. Expand
Homonojirimycin and N-methyl-homonojirimycin inhibit N-linked oligosaccharide processing.
In contrast to deoxynojirimycin, which has been reported to inhibit the formation of lipid-linked saccharides, no effects were seen on either the incorporation of mannose into lipid- linked saccharide, or the incorporate of leucine into protein. Expand
Purification and characterization of dolichyl-P-mannose:Man5(GlcNAc)2-PP-dolichol mannosyltransferase.
The enzyme was quite specific for dolichyl-P-mannose as the mannosyl donor and Man5(GlcNAc)2-PP-dolichol as themannosyl acceptor and gave a labeled disaccharide as the major product (greater than 90%). Expand
Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis. Relation to trehalose-based oligosaccharides attached to lipid.
Six different oligosaccharides were identified all with trehalose as the basic structure and additional glucose or galactose residues attached in various linkages, which may also have a protective role in mycobacteria, perhaps during latency. Expand