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Fibroin gene promoter contains a cluster of homeodomain binding sites that interact with three silk gland factors.
By nuclease protection and mobility shift assays, we have shown that the 5' flanking sequence of the Bombyx mori fibroin gene, which is known to be required for a maximal level of transcription in… Expand
Isolation and identification of the messenger RNA for silk fibroin from Bombyx mori.
Abstract The messenger RNA for the protein silk fibroin has been isolated from the posterior silk gland of Bombyx mori and identified by partial sequence analysis. The sequence of mRNA could be… Expand
Homeodomain binding sites in the 5' flanking region of the Bombyx mori silk fibroin light-chain gene.
Multiple A + T-rich stretches in the 5' flanking region of the Bombyx mori fibroin light-chain gene have been shown to bind two Drosophila homeodomain proteins, EVE (even-skipped) and ZEN… Expand
The genes for silk fibroin in Bombyx mori.
The genes for the protein silk fibroin were quantitated by hybridizaton of purified fibroin messenger RNA with the DNA from several tissues of the silk-worm Bombyx mori. Because of its high G + C… Expand
Quantitative measurements of fibroin messenger RNA synthesis in the posterior silk gland of normal and mutant Bombyx mori.
Abstract The amount of newly synthesized and accumulated fibroin messenger RNA has been measured quantitatively at various stages of posterior silk gland development in Bombyx mori. The two-step… Expand
Accentuated expression of silk fibroin genes in vivo and in vitro.
Fibroin mesenger RNA accumulates in the posterior silk gland of Bombyx mori at an almost constant rate (7 to 10 molecules/gene per min) throughout the fifth larval instar. During this period… Expand
Characterization of a tandemly repeated DNA sequence family originally derived by retroposition of tRNA(Glu) in the newt.
- S. Nagahashi, H. Endoh, Y. Suzuki, N. Okada
- Biology, Medicine
- Journal of molecular biology
- 20 November 1991
A previous report from this laboratory showed that in vitro transcription of total genomic DNA of the newt Cynopus pyrrhogaster resulted in a discrete sized 8 S RNA, which represented highly… Expand
The purification of the messenger RNA for silk fibroin.
Publisher Summary The messenger RNA (mRNA) that codes for the protein silk fibroin can be purified from the posterior gland of the silkworm Bombyx mori. The purification involves three sucrose… Expand