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Formins regulate the actin-related protein 2/3 complex-independent polarization of the centrosome to the immunological synapse.
Without Arp2/3-mediated actin nucleation, stimulated T cells could not form an F-actin-rich lamellipod, but instead produced polarized filopodia-like structures, and formins are essential cytoskeletal regulators of centrosome polarity in T cells. Expand
Production of human cells expressing individual transferred HLA-A,-B,-C genes using an HLA-A,-B,-C null human cell line.
The ability of mutant .221 to express quantitatively normal amounts of transferred class I genes suggests that it can serve as a recipient for, and then express, any cloned HLA-A, B, or -C gene that would normally be expressible in human B-lymphoblastoid cells. Expand
Coupling of the TCR to Integrin Activation by SLAP-130/Fyb
Data indicate that SLAP-130/Fyb is important for coupling TCR-mediated actin cytoskeletal rearrangement with activation of integrin function, and for T cells to respond fully to activating signals. Expand
The WAVE2 Complex Regulates Actin Cytoskeletal Reorganization and CRAC-Mediated Calcium Entry during T Cell Activation
This work shows that WAVE2 is a key component of the actin regulatory machinery in T cells and that it also participates in linking intracellular calcium store depletion to calcium release-activated calcium (CRAC) channel activation. Expand
The LFA-1 ligand ICAM-1 provides an important costimulatory signal for T cell receptor-mediated activation of resting T cells.
The present data demonstrate that LFA-1/ICAM-1 interaction is a potent costimulus for TCR-mediated activation; this observation, interpreted in light of previous reports, suggests that L FA-1 /ICam-1 is of major physiologic importance as a costimulatory signal. Expand
Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.
Observations clearly show that these three cloned, nonallelic, non-HLA-A, B, C class I genes encode alpha chains that can be expressed in human cells. Expand
Lymphocyte interactions with endothelial cells.
The molecules that mediate lymphocyte-endothelial cell adhesion are identified, the underlying principles of lymphocyte migration are described, and a model of the sequence of events that allow a lymphocyte to successfully attach to endothelium and migrate into the surrounding tissue is discussed. Expand
Regulated expression and binding of three VLA (β1) integrin receptors on T cells
The regulated binding of resting CD4+ human T cells to ECM through three VLA integrins is described, including a novel pathway of VLA-6 binding to laminin (LN). Expand
Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow.
Using the ImageStream imaging flow cytometer, a novel method is developed that measures nuclear translocation in large populations using cross-correlation analysis of nuclear and NF-kappaB images from each cell, and was successfully employed to measure IRF-7 translocated in plasmacytoid dendritic cells obtained from human peripheral blood. Expand
β-Actin specifically controls cell growth, migration, and the G-actin pool
Targeted deletion of Actb demonstrates that the β-actin gene, in contrast to the γ-actin gene, is an essential gene uniquely required for cell growth and migration. Cell motility and growth defectsExpand