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Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
TLDR
A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.
PropaneMonooxygenase and NAD+-Dependent Secondary AlcoholDehydrogenase in Propane Metabolism by Gordonia sp.StrainTY-5
TLDR
Northern blot analysis results and growth properties of a disrupted mutant indicate that Adh1 plays a major role in propane metabolism, and it is concluded that Gordonia sp.
Modification of a ubiquitin-like protein Paz2 conducted micropexophagy through formation of a novel membrane structure.
TLDR
It is shown that Paz2 and its modification system conduct micro Apexophagy through formation of the membrane structure, which explains the convergence between micropexophagy and macroautophagy with regard to de novo membrane formation.
PI4P-signaling pathway for the synthesis of a nascent membrane structure in selective autophagy
TLDR
The role of the PI4P-signaling pathway in selective autophagy is revealed, a process comprising multistep molecular events that lead to the de novo membrane formation.
Paz2 and 13 other PAZ gene products regulate vacuolar engulfment of peroxisomes during micropexophagy
Background: In the methylotrophic yeast Pichia pastoris, peroxisomes can be selectively degraded through direct engulfment by the vacuole in a process known as micropexophagy, but the mechanism of
Anaerobic Degradation of Aromatic Compounds by Magnetospirillum Strains: Isolation and Degradation Genes
TLDR
It was confirmed that the genus Magnetospirillum represents the third genus of denitrifying bacteria capable of degrading aromatic compounds under anaerobic conditions, besides the genera Thauera and Azoarcus.
Aerobic and Anaerobic Toluene Degradation by a Newly Isolated Denitrifying Bacterium, Thauera sp. Strain DNT-1
TLDR
It is concluded that strain DNT-1 modulates the expression of two different initial pathways of toluene degradation according to the availability of oxygen in the environment.
Gene Structures and Regulation of the Alkane Hydroxylase Complex in Acinetobacter sp. Strain M-1
TLDR
Two alkane hydroxylase complexes are switched by controlling the expression of twon-alkane hydoxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubred toxin ruductase are encoded by a single gene and expressed constitutively.
Peroxisome Degradation by Microautophagy in Pichia pastoris: Identification of Specific Steps and Morphological Intermediates
TLDR
These studies indicate that the microautophagic degradation of peroxisomes proceeds via specific intermediates, whose generation and/or processing is controlled by PAG gene products, and shed light on the poorly understood phenomenon of per oxisome homeostasis.
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