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Covalent immobilization of aminoacylase to alginate for L-phenylalanine production.
TLDR
This simple method provides irreversible immobilization of aminoacylase to give a biocatalyst with good operational stability and enhanced activity, and was more stable over a broader pH range.
Immobilization of aminoacylase by encapsulation in poly-L-lysine-stabilized calcium alginate beads.
TLDR
Aminoacylase I encapsulated in calcium alginate beads stabilized with poly-L-lysine was used for the production of L-phenylalanine by the hydrolysis of a racemic mixture of N-acetyl-DL-phenYLalanine and retained high biological activity.
Studies of L‐phenylalanine production by immobilised aminoacylase in stabilized calcium alginate beads
TLDR
Chitosan-coated calcium alginate beads was found to be the best among the immobilised systems studied and the activity and the optimum temperature of immobilised aminoacylase were higher than those of the free enzyme.
Kinetics of aspartame precursor synthesis catalysed by Pseudomonas aeruginosa elastase
Elastase isolated from Pseudomonas aeruginosa IFO 3455 was found to be an efficient protease to catalyse the synthesis of N-benzyloxycarbonyl-aspartylphenylalanine methyl ester, the precursor of the
Immobilization of aminoacylase in polyethyleneimine stabilized calcium alginate beads for L-phenylalanine production.
TLDR
Polyethyleneimine treatment was found to enhance the operational stability of the enzyme though its activity was substantially reduced and it was stable for at least 25 days under continuous operation without appreciable loss of activity.
Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
SummaryThe synthesis of the dipeptideN-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied.
L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
TLDR
The aims of this study were to establish an efficient immobilisation method for aminoacylase, and to report the preparation, properties, and applicability of the resulting immobilised aminoacyLase.
Effects of methanol on the synthesis of aspartame precursor catalysed byPseudomonas aeruginosa elastase
SummaryPseudomonas elastase was found to be efficient in catalysing the reaction betweenN-benzyloxycarbonyl-L-aspartic acid and L-phenylalanine methyl ester producing the aspartame precursor in