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Biotechnological production and applications of coenzyme Q10
The rational design of metabolic pathways in combination with engineering optimization of fermentation processes could facilitate the development of viable bioconversion processes.
Optimization of fed-batch fermentation for xylitol production by Candida tropicalis
Xylitol, a functional sweetener, was produced from xylose by biological conversion using Candida tropicalis ATCC 13803 by using the mass balance equations and kinetic parameters involved in the equations in order to increase finalxylitol concentration without affecting xylitol and productivity.
Screening and characterization of a novel esterase from a metagenomic library.
It is demonstrated that functional screening combined with the sequential uses of restriction enzymes to exclude already known enzymes is a useful approach for isolating novel enzymes from a metagenome.
Scale-up of erythritol production by an osmophilic mutant of Candida magnoliae
Erythritol production by an osmophilic mutant of Candida magnoliae was performed in fermentations of up 50 l to develop an optimized commercial process. By simultaneous feeding glucose and yeast
Purification and Characterization of a Novel Erythrose Reductase from Candida magnoliae
This enzyme had a dual coenzyme specificity with greater catalytic efficiency with NADH than with NADPH, and is specific for transferring the 4-pro-R hydrogen of NADH, which is typical of members of the aldo/keto reductase superfamily.
Kinetic studies on glucose and xylose transport in Saccharomyces cerevisiae
It was suggested that expression of the high-affinity transporter and increased affinity of glucose transporters forxylose under low glucose condition would provide a fermentation strategy for enhancing the productivity of xylitol by recombinant S. cerevisiae harboring the xylose reductase gene.
Batch and fed-batch production of coenzyme Q10 in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans
Analysis of the cell extract in recombinant E. coli BL21/pACDdsA by high performance liquid chromatography and mass spectrometry showed that CoQ10 rather than endogenous CoQ8 was biologically synthesized as the major coenzyme Q.
Elevation of glucose 6-phosphate dehydrogenase activity increases xylitol production in recombinant Saccharomyces cerevisiae
To increase the NAD(P)H-dependent xylitol production in recombinant Saccharomyces cerevisiae harboring the xylose reductase gene from Pichia stipitis, the activity of glucose 6-phosphate
Molecular cloning and biochemical characterization of a novel erythrose reductase from Candida magnoliae JH110
The genetic sequence and functional characteristics of a novel NADPH-dependent ER from C. magnoliae JH110 ER with high activity and catalytic efficiency would be very useful for in vitro erythritol production and could be applied for the production of eryTHritol in other microorganisms, which do not produce erystritol.
Screening, production and properties of a stereospecific esterase from Pseudomonas sp. S34 with high selectivity to (S)-ketoprofen ethyl ester
Abstract To isolate novel strains expressing an esterase that hydrolyzed the rac -ketoprofen ethyl ester to ( S )-ketoprofen in the stereospecific manner, we screened broad ecological niches and soil