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Treatment of porcine oocytes with MEM vitamins during in vitro maturation improves subsequent blastocyst development following nuclear transfer.
TLDR
Adding MEM vitamins to IVM medium could improve subsequent blastocyst development of porcine NT embryos in vitro, and addition of low concentrations of MEM vitamins only to maturation medium increased (P<0.05) the proportion of NT embryos developing into blastocysts compared with the control group. Expand
Brief exposure to cycloheximide prior to electrical activation improves in vitro blastocyst development of porcine parthenogenetic and reconstructed embryos.
TLDR
Brief exposure to cycloheximide prior to electrical activation may increase the subsequent blastocyst development rates in porcine parthenogenetic and reconstructed embryos. Expand
Streptolysin-o treatment of fetal fibroblasts improves cell fusion and in vitro development of porcine nuclear transfer embryos.
TLDR
Investigation of the effects of SLO treatment in donor cells that generate pores in the plasma membrane during in vitro development of porcine reconstructed embryos shows that SLO-mediated permeabilization of Porcine fetal fibroblast cells appears to improve the fusion rates and in vitro developed embryos. Expand
In Vitro Development of Interspecies Nuclear Transfer Embryos using Porcine Oocytes with Goat and Rabbit Somatic Cells
TLDR
The results suggest that goat and bovine fetal fibroblasts were less efficacious than porcine‐porcine cloned embryos and that culture condition could be an important factor in iSCNT. Expand
70 FETAL FIBROBLAST CELLS PERMEABILIZED WITH STREPTOLYSIN O IMPROVE THE RATES OF FUSION AND IN VITRO DEVELOPMENT OF PORCINE RECONSTRUCTED EMBRYOS
TLDR
In conclusion, permeabilization of porcine fetal fibroblast cells with SLO improves the fusion rates and in vitro development of Porcine reconstructed embryos and there was no significant difference in the total cell number of blastocysts among SLO-treated groups. Expand
EFFECT OF FIBRIN ON MOUSE EMBRYO DEVELOPMENT IN VITRO
TLDR
The data demonstrate that fibrin glue have no toxicity on mouse embryo development and are suitable for interaction study between the embryotrophic factor and development embryos. Expand