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Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17
Abstract Two thermophilic, anaerobic bacteria named JN4 and GD17 were isolated from rotten wheat straw. The cellulolytic bacterium JN4 was identified as Clostridium thermocellum and its companion… Expand
Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism
BackgroundNon-productive cellulase adsorption onto lignin has always been deemed to negatively affect the enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding enzyme-lignin… Expand
Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum
Filamentous fungus Penicillium oxalicum produces diverse lignocellulolytic enzymes, which are regulated by the combinations of many transcription factors. Here, a single-gene disruptant library for… Expand
Comparative Genomic Analysis of dha Regulon and Related Genes for Anaerobic Glycerol Metabolism in Bacteria
The dihydroxyacetone ( dha) regulon of bacteria encodes genes for the anaerobic metabolism of glycerol. In this work, genomic data are used to analyze and compare the dha regulon and related genes in… Expand
Genomic and Secretomic Analyses Reveal Unique Features of the Lignocellulolytic Enzyme System of Penicillium decumbens
Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and… Expand
Directed evolution for engineering pH profile of endoglucanase III from Trichoderma reesei.
The potential of cellulase has been revealed not only in biomass conversion but also in various industrial processes, including food, textiles, laundry, pulp, and paper. Due to the need for… Expand
Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability.
- Yuqi Qin, X. Wei, Xiangmei Liu, T. Wang, Y. Qu
- Biology, Medicine
- Protein expression and purification
- 1 March 2008
Cel5A (endoglucanase II) of Trichoderma reesei was expressed in Saccharomyces cerevisiae then purified. Two components (C1 and C2) of recombinant Cel5A with different glycosylation were obtained.… Expand
Isolation and characterization of a β-glucosidase from Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulase supplementation.
- Mei Chen, Yuqi Qin, Ziyong Liu, K. Liu, Fengshan Wang, Y. Qu
- Chemistry, Medicine
- Enzyme and microbial technology
- 5 May 2010
A β-glucosidase from Penicillium decumbens was purified and characterized. The enzyme presented as a single band of 120kDa on SDS-PAGE, showed optimal temperature of 65-70°C and optimal pH of… Expand
Simultaneous saccharification and fermentation of acid-pretreated corncobs with a recombinant Saccharomyces cerevisiae expressing β-glucosidase
Abstract To reduce the cellobiose inhibition of exoglucanase and endogulcanase and enhance cellulose hydrolysis during simultaneous saccharification and fermentation (SSF), a β-glucosidase encoding… Expand
High concentration ethanol production from corncob residues by fed-batch strategy.
Ethanol production from corncob residues (CCR) pretreated by different methods was studied. The structure features of these CCR were analyzed by Fourier transform-infrared spectrum (FT-IR), X-ray… Expand