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Molecular and cytogenetic evidence of three sibling species of the Anopheles barbirostris Form A (Diptera:Culicidae) in Thailand
TLDR
Asynaptic regions along the five polytene chromosome arms of F1-hybrid larvae clearly supported the existence of three sibling species within A. barbirostris Form A, provisionally named species A1, A2, and A3.
Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand
Seventeen isolines of Anopheles barbirostris derived from animal-biting female mosquitoes showed three karyotypic forms: Form A (X2, Y1) in five isolines from Phetchaburi province; Form B (X1, X3,
Molecular identification of infective larvae of three species of Onchocerca found in wild-caught females of Simulium bidentatum in Japan.
TLDR
The present study strongly suggests that S. bidentatum plays a role as a vector in the transmission of zoonotic onchocerciasis due to O. dewittei japonica in Japan.
Blackfly vectors of zoonotic onchocerciasis in Japan
TLDR
Molecular identification of infective larvae found in wild‐caught female blackflies showed that Simulium bidentatum (Shiraki) (Diptera: Simuliidae) is a natural vector of O. dewittei japonica, and also Onchocerca sp.
New zoonotic cases of Onchocerca dewittei japonica (Nematoda: Onchocercidae) in Honshu, Japan
TLDR
Findings indicate the spread of a zoonosis caused by O. dewittei japonica in the western part of Honshu, where wild boars have recently extended their habitats because of decreased annual snowfall, unused rice fields and a decline in the number of hunters in Japan.
Detection of Orientia tsutsugamushi (Rickettsiales: Rickettsiaceae) in Unengorged Chiggers (Acari: Trombiculidae) from Oita Prefecture, Japan, by Nested Polymerase Chain Reaction
TLDR
Serotypes of O. tsutsugamushi were identified by restriction fragment-length polymorphism (RFLP) analysis and indicate that the disease was widely distributed in various trombiculid species, even in the nonendemic area.
Development of a multiplex PCR assay for the identification of eight species members of the Thai Hyrcanus Group (Diptera: Culicidae)
TLDR
This study first developed a simple and robust multiplex PCR assay, based on second internal transcribed spacer sequences of ribosomal DNA, for differentiating the eight species members of the Thai Hyrcanus Group.
An effective method for the identification and separation of Anopheles minimus, the primary malaria vector in Thailand, and its sister species Anopheles harrisoni, with a comparison of their mating
TLDR
This study is the first to examine and discover the usefulness of large sensilla coeloconica on the antennae of females and the frequency of clasper movement in males for distinguishing the sibling species An.
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