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[Detection of Pneumocystis carinii with DNA amplification].
cDNA sequence diversity and genomic clusters of major surface glycoprotein genes of Pneumocystis carinii.
TLDR
The cDNAs encoding MSG antigens were cloned from a lambda gt11 expression library of rat-derived P. carinii by immunoscreening and have significant sequence homology to tropomyosins and myosins, suggesting physical or functional association with the membrane cytoskeleton. Expand
Antigenic variation by positional control of major surface glycoprotein gene expression in Pneumocystis carinii.
Major cell surface glycoproteins (MSGs) of Pneumocystis carinii play a crucial role in host-parasite interactions during P. carinii pneumocystosis in AIDS patients. Genes encoding MSGs are repeatedExpand
TNF‐a mediates the development of anaemia in a murine Trypanosoma brucei rhodesiense infection, but not the anaemia associated with a murine Trypanosoma congolense infection
TLDR
Data suggest that more than one mechanism promotes the development of anaemia associated with trypanosomiasis, and that tumour necrosis factor‐α, produced by activated macrophages, is one of them. Expand
Unique telomeric expression site of major-surface-glycoprotein genes of Pneumocystis carinii.
  • M. Wada, Y. Nakamura
  • Biology, Medicine
  • DNA research : an international journal for rapid…
  • 1996
TLDR
It is shown, by BAL-31 exonuclease cleavage and DNA cloning experiments, that the unique expression site (previously termed UCS) of MSG genes located in the 500-kb chromosome is telomeric. Expand
Prevalence of Bartonella henselae, Bartonella clarridgeiae and the 16S rRNA gene types of Bartonella henselae among pet cats in Japan.
TLDR
The authors investigated bacteriologically the prevalence of Bartonella infection among 690 pet cats derived from 10 private animal hospitals in six cities and 4 counties of Japan and found that B. henselae among cats in Japan was demonstrated for the first time. Expand
Glycoproteins composed of major surface immunodeterminants of Pneumocystis carinii
TLDR
The majority of sera tested from humans with diagnosed pneumocystosis reacted strongly with the P115 proteins, suggesting that the isoelectric variants may be due primarily to differences in glycosylation. Expand
MSG gene cluster encoding major cell surface glycoproteins of rat Pneumocystis carinii.
  • M. Wada, Y. Nakamura
  • Biology, Medicine
  • DNA research : an international journal for rapid…
  • 1994
TLDR
Results strongly suggested that diverse MSG genes are clustered in tandem repeats and these clusters involve ORF-3, which seemed to be an anchoring sequence in the cell membrane. Expand
An evaluation of the agar plate method for the detection of Strongyloides stercoralis in northern Thailand.
TLDR
The agar plate method appeared to be more efficient than traditional methods for detecting Strongyloides in human stool samples, and other positive factors in its favour were ease of manipulation and low cost. Expand
cDNA Cloning and Overexpression of Cell Surface Subtilisin‐Like Proteases (SSP) of Pneumocystis carinii
  • M. Wada, Y. Nakamura
  • Medicine, Biology
  • The Journal of eukaryotic microbiology
  • 1 November 1997
TLDR
The nucleotide sequence analyses of ten ORF3-cDNA clones isolated in this study revealed that they are similar but not identical, showing polymorphism in the overlapped sequences, consistent with the previous finding that the OW3 DNA probe hybridized to multiple restriction fragments. Expand
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