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Mammalian valyl‐tRNA synthetase forms a complex with the first elongation factor
The high‐molecular‐mass form of valyl‐tRNA synthetase is associated with the first elongation factor activity. It includes two polypeptides of about 50 kDa and two others of 40 and 30 kDa, identified… Expand
Purification and properties of a high-molecular-mass complex between Val-tRNA synthetase and the heavy form of elongation factor 1 from mammalian cells.
- Y. Motorin, A. Wolfson, D. Loehr, A. F. Orlovsky, K. Gladilin
- Biology, Medicine
- European journal of biochemistry
- 1 October 1991
In extracts of various mammalian tissues obtained in the presence of protease inhibitors Val-tRNA synthetase exists exclusively as a complex with a molecular mass of about 800 kDa. This complex was… Expand
Purification of valyl‐tRNA synthetase high‐molecular‐mass complex from rabbit liver
A high‐molecular‐mass complex containing valyl‐tRNA synthetase has been purified to homogeneity from rabbit liver. The molecular mass of the complex is about 800 kDa. The complex consists of four… Expand
Anticodon‐dependent aminoacylation of RNA minisubstrate by lysyl‐tRNA synthetase
Specific inhibition of mammalian lysyl‐tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than… Expand
Purification of mammalian tyrosyl-tRNA synthetase by high-performance liquid chromatography.
Rapid isolation of Escherichia coli β-gatactosidase by fast protein liquid chromatography
Crucial role of pyrophosphate in the aminoacylation of E. coli tRNAPhe by yeast phenylalanyl‐tRNA synthetase
Rapid inactivation of the yeast phenylalanyl‐tRNA synthetase in the course of aminoacylation of the heterologous E. coli tRNAPhe is observed. This inactivation occurs due to the formation of the… Expand
Determination of binding constants by high-performance gel filtration.
- Y. Motorin
Gel filtration is one of the most commonly used non-kinetic methods for the determination of association constants of ligands with proteins and nucleic acids1-4. The most widely used modification of… Expand