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Cx32 but not Cx26 is associated with tight junctions in primary cultures of rat hepatocytes.
On freeze-fracture replicas, gap junctions are frequently colocalized with tight junctions. In this study, to elucidate the relationship between gap- and tight-junction proteins, we investigated theExpand
Reconstruction of hepatic organoid by rat small hepatocytes and hepatic nonparenchymal cells
Hepatic cells isolated from an adult rat liver, consisting of small hepatocytes (SHs), mature hepatocytes (MHs), liver epithelial cells (LECs), Kupffer cells, sinusoidal endothelial cells, andExpand
Reappearance and long-term maintenance of connexin32 in proliferated adult rat hepatocytes: use of serum-free L-15 medium supplemented with EGF and DMSO.
Intercellular communication, especially gap junctional communication, is thought to be one of the highly differentiated functions of hepatocytes. In primary cultures of rat hepatocytes, it has beenExpand
Cx32 formation and/or Cx32-mediated intercellular communication induces expression and function of tight junctions in hepatocytic cell line.
Gap junctional intercellular communication (GJIC) is thought to play a crucial role in cell differentiation. Small gap junction plaques are frequently associated with tight junction strands inExpand
Proliferation of rat small hepatocytes after long-term cryopreservation.
BACKGROUND/AIMS The demand for clinical use of hepatocytes is escalating because cell transplantation will be an alternative to orthotopic liver transplantation and the shortage of liver donors is aExpand
Redifferentiation of proliferated rat hepatocytes cultured in L15 medium supplemented with EGF and DMSO
SummaryPrimary adult rat hepatocytes were cultured in serum-free L15 medium supplemented with 20 mM NaHCO3 and 10 ng/ml epidermal growth factor in a 5% CO2:95% air incubator. The number of cellsExpand
In hepatocytes of fetal rats, cytoplasmic organelles identifiable as microbodies appeared, although only a few of them showed nucleoids and most of them generally had an electronlucent appearance dueExpand
The nucleoids of rat liver cell microbodies. Fine structure and enzymes.
The nucleoids of microbodies of rat liver cells were isolated in a highly homogeneous and pure state, by treating the microbody-rich fraction, prepared from 10% polyvinylpyrrolidone-0.25 M sucroseExpand
Growth and maturation of small hepatocytes isolated from adult rat liver.
Small hepatocytes existed in the supernatant following low-speed centrifugation of the cell suspension after collagenase liver perfusion. The cells proliferated for more than 2 months and formedExpand
Induction of tight junctions in human connexin 32 (hCx32)-transfected mouse hepatocytes: connexin 32 interacts with occludin.
Small gap junction plaques are associated with tight junction strands in some cell types including hepatocytes and it is thought that they may be closely related to tight junctions and theExpand