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Hairpin Opening and Overhang Processing by an Artemis/DNA-Dependent Protein Kinase Complex in Nonhomologous End Joining and V(D)J Recombination
Mutations in the Artemis protein in humans result in hypersensitivity to DNA double-strand break-inducing agents and absence of B and T lymphocytes (radiosensitive severe combined immune deficiencyExpand
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Mechanism and regulation of human non-homologous DNA end-joining
Non-homologous DNA end-joining (NHEJ) — the main pathway for repairing double-stranded DNA breaks — functions throughout the cell cycle to repair such lesions. Defects in NHEJ result in markedExpand
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Delineation of Joint Molecule Resolution Pathways in Meiosis Identifies a Crossover-Specific Resolvase
At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution inExpand
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A biochemically defined system for mammalian nonhomologous DNA end joining.
Nonhomologous end joining (NHEJ) is a major pathway in multicellular eukaryotes for repairing double-strand DNA breaks (DSBs). Here, the NHEJ reactions have been reconstituted in vitro by usingExpand
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The DNA-dependent Protein Kinase Catalytic Subunit Phosphorylation Sites in Human Artemis*
Artemis protein has irreplaceable functions in V(D)J recombination and nonhomologous end joining (NHEJ) as a hairpin and 5′ and 3′ overhang endonuclease. The kinase activity of the DNA-dependentExpand
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Temporally and biochemically distinct activities of Exo1 during meiosis: double-strand break resection and resolution of double Holliday junctions.
The Rad2/XPG family nuclease, Exo1, functions in a variety of DNA repair pathways. During meiosis, Exo1 promotes crossover recombination and thereby facilitates chromosome segregation at the firstExpand
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Severe combined immunodeficiency and microcephaly in siblings with hypomorphic mutations in DNA ligase IV
DNA double‐strand breaks (dsb) during V(D)J recombination of T and B lymphocyte receptor genes are resolved by the non‐homologous DNA end joining pathway (NHEJ) including at least six factors: Ku70,Expand
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Functional and biochemical dissection of the structure‐specific nuclease ARTEMIS
During V(D)J recombination, the RAG1 and RAG2 proteins form a complex and initiate the process of rearrangement by cleaving between the coding and signal segments and generating hairpins at theExpand
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Omenn syndrome due to ARTEMIS mutations.
Omenn syndrome (OS) is characterized by severe combined immunodeficiency (SCID) associated with erythrodermia, hepatosplenomegaly, lymphadenopathy, and alopecia. In patients with OS, B cells areExpand
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The Artemis:DNA-PKcs endonuclease cleaves DNA loops, flaps, and gaps.
In eukaryotic cells, nonhomologous DNA end joining (NHEJ) is a major pathway for repair of double-strand DNA breaks (DSBs). Artemis and the 469kDa DNA-dependent protein kinase (DNA-PKcs) togetherExpand
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