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Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase
- Tao Xu, Y. Li, +6 authors J. Zhou
- Biology, Medicine
- Applied and Environmental Microbiology
- 24 April 2015
ABSTRACT The CRISPR-Cas9 system is a powerful and revolutionary genome-editing tool for eukaryotic genomes, but its use in bacterial genomes is very limited. Here, we investigated the use of the… Expand
One-step label-free optical genosensing system for sequence-specific DNA related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods.
A one-step label-free optical genosensing method has been developed in this contribution by taking short DNA target with its sequence related to the human immunodeficiency virus type 1 (HIV-1) as an… Expand
Carbon nanotubes as a low background signal platform for a molecular aptamer beacon on the basis of long-range resonance energy transfer.
Although holding the advantages of both an aptamer and a molecular beacon (MB), a molecular aptamer beacon (MAB) needs complicated and expensive modifications at both of its ends and usually has a… Expand
Synergistic antiviral effect of curcumin functionalized graphene oxide against respiratory syncytial virus infection.
The diseases attributable to viruses remain a global burden. The respiratory syncytial virus (RSV), which is considered as the major viral pathogen of the lower respiratory tract of infants, has been… Expand
Optimized stereo reconstruction of free-form space curves based on a nonuniform rational B-spline model.
- Yi Jun Xiao, Y. Li
- Computer Science, Medicine
- Journal of the Optical Society of America. A…
- 1 September 2005
Analytical reconstruction of 3D curves from their stereo images is an important issue in computer vision. We present an optimization framework for such a problem based on a nonuniform rational… Expand
Visual observation of the mercury-stimulated peroxidase mimetic activity of gold nanoparticles.
- Y. Long, Y. Li, Y. Liu, J. Zheng, Jie Tang, C. Huang
- Chemistry, Medicine
- Chemical communications
- 25 October 2011
Mercury-stimulated peroxidase mimetic activity of gold nanoparticles was presented, with which a sensitive label-free colorimetric method for Hg(2+) was developed.
A nanosized metal-organic framework of Fe-MIL-88NH₂ as a novel peroxidase mimic used for colorimetric detection of glucose.
In this paper, a nanosized porous metal-organic framework, Fe-MIL-88NH₂, was facilely prepared with a uniform octahedral shape by the addition of acetic acid, and for the first time was demonstrated… Expand
One-step synthesis of fluorescent hydroxyls-coated carbon dots with hydrothermal reaction and its application to optical sensing of metal ions
Carbon dots (CDs) with average diameter of 3.1 ± 0.5 nm were facilely synthesized with candle soot through hydrothermal reaction in sodium hydroxide aqueous solution. The as-prepared CDs were covered… Expand
A spectrophotometric study on the interaction of neutral red with double-stranded DNA in large excess.
With the measurement of molecular absorption, the interaction of neutral red (NR) with double-stranded DNA in large excess was investigated. It was found that the interaction of NR, existing in the… Expand
A visual detection of hydrogen peroxide on the basis of Fenton reaction with gold nanoparticles.
- You Sang, L. Zhang, Y. Li, L. Chen, J. Xu, C. Huang
- Chemistry, Medicine
- Analytica chimica acta
- 5 February 2010
Nowadays, hydrogen peroxide (H(2)O(2)) has attracted more and more attentions in biochemical fields owing to its important role in biological systems. In this contribution, we propose a novel assay… Expand