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Single‐cell analysis reveals cancer stem cell heterogeneity in hepatocellular carcinoma
Intratumor molecular heterogeneity of hepatocellular carcinoma is partly attributed to the presence of hepatic cancer stem cells (CSCs). Different CSC populations defined by various cell surface… Expand
Robustness of RNA sequencing on older formalin-fixed paraffin-embedded tissue from high-grade ovarian serous adenocarcinomas
Formalin-fixed paraffin-embedded (FFPE) tissues are among the most widely available clinical specimens. Their potential utility as a source of RNA for transcriptome studies would greatly enhance… Expand
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples.
- Y. Levin, Keyur Talsania, B. Tran, J. Shetty, Yongmei Zhao, M. Mehta
- Computer Science, Medicine
- Journal of visualized experiments : JoVE
- 8 June 2020
We describe the various steps for sample QC, library preparation and QC, sequencing, and data analysis that can help to increase the amount of useful data obtained from low quality RNA, such as that obtained from FFPE-RNA tissues. Expand