• Publications
  • Influence
A DNA transfection system for generation of influenza A virus from eight plasmids.
TLDR
An eight-plasmid DNA transfection system for the rescue of infectious influenza A virus from cloned cDNA facilitates the design and recovery of both recombinant and reassortant influenza A viruses, and may also be applicable to the recovery of other RNA viruses entirely from cloning cDNA.
Generation of influenza A viruses entirely from cloned cDNAs.
TLDR
A new reverse-genetics system that allows one to efficiently generate influenza A viruses entirely from cloned cDNAs is described, which should be useful in viral mutagenesis studies and in the production of vaccines and gene therapy vectors.
Molecular Basis for High Virulence of Hong Kong H5N1 Influenza A Viruses
TLDR
Using reverse genetics, it is shown that a mutation at position 627 in the PB2 protein influenced the outcome of infection in mice, and high cleavability of the hemagglutinin glycoprotein was an essential requirement for lethal infection.
Emergence and pandemic potential of swine-origin H1N1 influenza virus
TLDR
Efforts to control these outbreaks and real-time monitoring of the evolution of this virus should provide invaluable information to direct infectious disease control programmes and to improve understanding of the factors that determine viral pathogenicity and/or transmissibility.
Early Alterations of the Receptor-Binding Properties of H1, H2, and H3 Avian Influenza Virus Hemagglutinins after Their Introduction into Mammals
TLDR
The results show that the receptor-binding specificity of the HA is altered early after the transmission of an avian virus to humans and pigs and, therefore, may be a prerequisite for the highly effective replication and spread which characterize epidemic strains.
Molecular Basis for the Generation in Pigs of Influenza A Viruses with Pandemic Potential
TLDR
A structural basis for the hypothesis that pigs may serve as “mixing vessels” for the generation of human-avian influenza A virus reassortants, similar to those responsible for the 1957 and 1968 pandemics is demonstrated.
In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses
TLDR
It is shown that CA04 is sensitive to approved and experimental antiviral drugs, suggesting that these compounds could function as a first line of defence against the recently declared S-OIV pandemic.
Influenza: lessons from past pandemics, warnings from current incidents
TLDR
A review of molecular factors that contribute to the emergence of pandemic strains in poultry and in humans and the eradication of pathogenic avian influenza viruses is reviewed.
Avian flu: Influenza virus receptors in the human airway
TLDR
An anatomical difference in the distribution in the human airway of the different binding molecules preferred by the avian and human influenza viruses is demonstrated to provide a rational explanation for why H5N1 viruses at present rarely infect and spread between humans although they can replicate efficiently in the lungs.
Experimental adaptation of an influenza H5 haemagglutinin (HA) confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets
TLDR
Results indicate that H5 HA can convert to an HA that supports efficient viral transmission in mammals, and will help individuals conducting surveillance in regions with circulating H5N1 viruses to recognize key residues that predict the pandemic potential of isolate, which will inform the development, production and distribution of effective countermeasures.
...
1
2
3
4
5
...