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Fluorescence lifetime imaging of oxygen in living cells
The usefulness of the fluorescent probe ruthenium tris(2,2′-dipyridyl) dichloride hydrate (RTDP) for the quantitative imaging of oxygen in single cells was investigated utilizing fluorescenceExpand
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Quantitative pH imaging in cells using confocal fluorescence lifetime imaging microscopy.
The pH-sensitive probe carboxy SNAFL-1 can be used for imaging using ratiometric and fluorescence lifetime techniques. The former method suffers from the drawback that quantitative pH imaging inExpand
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Fluorescence lifetime imaging using a confocal laser scanning microscope
The implementation of a fast fluorescence life-time imaging method in a confocal laser scanning microscope is described. The set up utilizes a low-power continuous wave (CW) argon ion laser equippedExpand
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DPH is localised in two distinct populations in lipid vesicles
Abstract The fluorescence lifetime and anisotropy of DPH molecules in vesicles of lipids is commonly interpreted in terms of intrinsic structural heterogeneities of the bilayer. However, theseExpand
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Slow-motion ESR study of order and dynamics in oriented lipid multibilayers: effects of unsaturation and hydration.
Electron spin resonance (ESR) experiments were carried out on 3-doxyl-5 alpha-cholestane spin-label (CSL) molecules embedded in macroscopically oriented multibilayers ofExpand
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Fluorescence lifetime imaging of free calcium in single cells
It is shown here that the intensity probe CalciumGreen is suitable for quantitative Ca2+ concentration determinations using fluorescence lifetime imaging. The probe is characterized by two distinctExpand
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CHLOROPHYLLS IN POLYMERS. I. STATE OF CHLOROPHYLL a IN UNSTRETCHED POLYMER SYSTEMS
Abstract— Model systems for the study of energy transfer processes are useful for the elucidation of the various factors governing the mechanism of energy transfer in photosynthetic systems. Here weExpand
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Study of the orientational ordering of carotenoids in lipid bilayers by resonance-Raman spectroscopy.
The orientational ordering of beta-carotene and crocetin embedded in lamellar model membranes has been investigated by angle-resolved resonance Raman scattering at a temperature well above the phaseExpand
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The correlation between molecular orientational order and reorientational dynamics of probe molecules in lipid multibilayers
The behaviour of probe molecules in lipid systems can be characterized in terms of molecular order parameters and rates of reorientational motion. The correlation between these parameters has beenExpand
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Effects of steroid molecules on the dynamical structure of dioleoylphosphatidylcholine and digalactosyldiacylglycerol bilayers.
The ESR spectra of cholestane spin labels (CSL) in dioleoylphosphatidylcholine (DOPC) bilayers containing 20 wt% of cholesterol, 7-dehydrocholesterol, beta-sitosterol, stigmasterol and lanosterolExpand
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