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PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins.
TLDR
In vitro analysis showed that PER1 is required for the production of lyso-GPI, suggesting that Per1p possesses or regulates the GPI-phospholipase A2 activity, and human PERLD1 is a functional homologue of PER1.
OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine‐linked oligosaccharides.
TLDR
The OCH1 gene was found to encode a novel mannosyltransferase which specifically transfers [14C]mannose to the unique acceptor, the core‐like oligosaccharide of cell wall mannan accumulated in the och1 disruptant.
Demonstration of mammalian protein O-mannosyltransferase activity: coexpression of POMT1 and POMT2 required for enzymatic activity.
TLDR
A method to detect protein O-mannosyltransferase activity in mammalian cells was developed and it was shown that coexpression of both PomT1 and POMT2 was necessary for the enzyme activity, but expression of either POM t1 or POMt2 alone was insufficient.
Mannosylphosphate transfer to yeast mannan.
Fatty acid remodeling of GPI-anchored proteins is required for their raft association.
TLDR
It is reported that GPI-APs, with two saturated fatty chains, are generated from those bearing an unsaturated chain by fatty acid remodeling that occurs most likely in the Golgi and requires post-GPI-attachment to proteins (PGAP)2 and PGAP3 and shows that the remodeling requires the preceding PGAP1-mediated deacylation from inositol of GPI -APs in the endoplasmic reticulum.
Functional Analysis of Arabidopsis thaliana RHM2/MUM4, a Multidomain Protein Involved in UDP-D-glucose to UDP-L-rhamnose Conversion*
TLDR
Direct evidence is presented that all three RHM proteins have UDP-d-glucose 4,6-dehydratase, UDP-4-keto- 6-deoxy- d- glucose 3,5-epimerase, and UDP-l-rhamnose 4-keti-reductase activities in the cytoplasm when expressed in the yeast Saccharomyces cerevisiae.
Molecular Cloning and Identification of 3′-Phosphoadenosine 5′-Phosphosulfate Transporter*
TLDR
A novel human gene encoding a PAPS transporter, which is named PAPST1, and the Drosophila melanogaster ortholog, slalom (sll), is identified and insights into the significance of PAPS transport and post-translational sulfation are provided.
Inositol deacylation by Bst1p is required for the quality control of glycosylphosphatidylinositol-anchored proteins.
TLDR
A mutant form of the beta-1,3-glucanosyltransferase Gas1p (Gas1*p) is constructed as a model misfolded GPI-anchored protein and its quality control mechanism is reported on.
Alg14 Recruits Alg13 to the Cytoplasmic Face of the Endoplasmic Reticulum to Form a Novel Bipartite UDP-N-acetylglucosamine Transferase Required for the Second Step of N-Linked Glycosylation*
TLDR
Results demonstrate that this novel UDP-GlcNAc transferase is a unique eukaryotic ER glycosyltransferase that is comprised of at least two functional polypeptides, one that functions in catalysis and the other as a membrane anchor.
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