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A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield
An HIV antibody achieves potency and breadth by binding simultaneously to two conserved glycans on the viral envelope protein. The HIV envelope (Env) protein gp120 is protected from antibodyExpand
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E3 ubiquitin ligase that recognizes sugar chains
N-glycosylation of proteins in the endoplasmic reticulum (ER) has a central role in protein quality control. Here we report that N-glycan serves as a signal for degradation by theExpand
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Substrate Specificity Analysis of Endoplasmic Reticulum Glucosidase II Using Synthetic High Mannose-type Glycans*
Glucosidase II (Glc'ase II) is a glycan-processing enzyme that trims two α1,3-linked Glc residues in succession from the glycoprotein oligosaccharide Glc2Man9GlcNAc2 to give Glc1Man9GlcNAc2 andExpand
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The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation.
Misfolded glycoproteins are translocated from the endoplasmic reticulum (ER) into the cytoplasm for proteasome-mediated degradation. OS-9 protein is thought to participate in ER-associatedExpand
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The sugar-binding ability of ERGIC-53 is enhanced by its interaction with MCFD2.
Combined deficiency of factors V and VIII (F5F8D) is a bleeding disorder caused by mutations in LMAN1 or MCFD2. LMAN1 encodes ERGIC-53, a cargo receptor with an L-type lectin domain, and MCFD2 is aExpand
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Analysis of the sugar‐binding specificity of mannose‐binding‐type Jacalin‐related lectins by frontal affinity chromatography – an approach to functional classification
The Jacalin‐related lectin (JRL) family comprises galactose‐binding‐type (gJRLs) and mannose‐binding‐type (mJRLs) lectins. Although the documented occurrence of gJRLs is confined to the familyExpand
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Sugar-binding Properties of VIP36, an Intracellular Animal Lectin Operating as a Cargo Receptor*
The vesicular integral protein of 36 kDa (VIP36) is an intracellular animal lectin that acts as a putative cargo receptor, which recycles between the Golgi and the endoplasmic reticulum. Although itExpand
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Glycerophospholipid regulation of modality-specific sensory axon guidance in the spinal cord
Axon paths in developing spinal cords Sensory neurons entering the spinal cord take different paths as inputs for pain and proprioception diverge. Working with chick and mouse embryos, Guy et al.Expand
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Identification and Characterization of an Intracellular Lectin, Calnexin, from Aspergillus oryzae Using N-Glycan-Conjugated Beads
Recently, asparagine-linked oligosaccharides (N-glycans) have been found to play a pivotal role in glycoprotein quality control in the endoplasmic reticulum (ER). In order to screen proteinsExpand
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High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors.
We describe here the construction of Gateway-compatible vectors, pBGP1-DEST and pPICZα-DEST, for rapid and convenient preparation of expression plasmids for production of secretory proteins in PichiaExpand
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