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Endo-β-N-acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol
TLDR
The identification of the gene encoding human cytosolic ENGase, a key enzyme involved in the processing of free oligosaccharides in the cytosol, was reported, suggesting that this enzyme is involved in basic biological processes in eukaryotic cells.
Purification and enzymatic properties of peptide:N-glycanase from C3H mouse-derived L-929 fibroblast cells. Possible widespread occurrence of post-translational remodification of proteins by
TLDR
It is proposed here that PNGase-catalyzed N-deglycosylation is a functionally important universal feature in living cells.
Characterization of the Major Core Structures of the α2→8-linked Polysialic Acid-containing Glycan Chains Present in Neural Cell Adhesion Molecule in Embryonic Chick Brains*
TLDR
The overall structure of α2→8-linked polySia chain-containing tri- and tetraantennary glycans present in the chick embryonic brain N-CAM molecule was examined and it is possible that the unusual structural features identified in this study might play a role in the initiation of polysialylation.
Site-specific de-N-glycosylation of diglycosylated ovalbumin in hen oviduct by endogenous peptide: N-glycanase as a quality control system for newly synthesized proteins.
TLDR
The present findings support the view that de-N-glycosylation catalyzed by PNGase may be involved in quality control of newly synthesized proteins by converting its diglycosylated form into the mono-N -glycosolated form that can be secreted.
An ultrasensitive chemical method for polysialic acid analysis.
TLDR
The new method was used to analyze the degree of polymerization of a polySia-containing glycopeptide fraction derived from embryonic chicken brain, and the results were compared with those obtained by HPAEC-PED.
Identification of peptide:N-glycanase activity in mammalian-derived cultured cells.
TLDR
This represents the first demonstration of PNGase in mammalian cells and thus PNGase appears to be a very common enzyme expressed in not only plants and bacteria but also a wide range of animals although its functional significance remains to be clarified.
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