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Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins
The measurement of nitric oxide (NO) is important for direct examination of the regulatory roles of NO in various biological systems. Diaminofluoresceins (DAFs), new fluorescence indicators for NO,Expand
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Purification and partial characterization of linoleoyl-CoA desaturase from rat liver microsomes.
Abstract The terminal enzyme of the linoleoyl-CoA desaturase system was purified from rat liver microsomes by Triton X-100 solubilization, DEAE-cellulose, CM-Sephadex, and affinity chromatography onExpand
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Nitric oxide production from macrophages is regulated by arachidonic acid metabolites.
In activated macrophages the inducible form of the enzyme nitric oxide (NO) synthase generates high amounts of the toxic mediator NO. After 20 h of treatment with LPS rat peritoneal macrophagesExpand
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Purification of squalene epoxidase from rat liver microsomes.
Abstract Squalene epoxidase was purified from rat liver microsomes by DEAE-cellulose, alumina C ν gel, hydroxylapatite, CM-Sephadex C-50 and Cibacron Blue Sepharose 4B in the presence of TritonExpand
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Cyclosporin A protects pancreatic islet cells from nitric oxide‐dependent macrophage cytotoxicity
It has been shown earlier in an in‐vitro model of inflammatory islet cell death that activated macrophages lyse islet cells via the release of nitric oxide. Here we report that cyclosporin AExpand
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Cytoplasmic location of linoleoyl-CoA desaturase in microsomal membranes of rat liver.
The intramembrane localization of linoleoyl-CoA desaturase in rat liver microsomes was examined by various methods, such as digestion by proteases, effect of detergents, and inhibition by theExpand
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Effects of dietary vitamin B-2 and vitamin E on the delta9-desaturase and catalase activities in the rat liver microsomes.
The effects of dietary vitamin B-2 and vitamin E on delta9-desaturation of stearoyl-CoA, catalase, glutathione peroxidase, superoxide dismutase and electron transport components in rat liverExpand
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Purification and characterization of cytosolic liver protein facilitating heme transport into apocytochrome b5 from mitochondria. Evidence for identifying the heme transfer protein as belonging to a
The results of the present experiment are shown in terms of the transport of protoheme from mitochondria to apocytochrome b5 when fresh rat liver mitochondria, apocytochrome b5, and cytosol wereExpand
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Enhanced production of platelet-activating factor in stimulated rat leukocytes pretreated with triacsin C, A novel acyl-coA synthetase inhibitor.
Triacsin C, a product of Streptmyces sp. SK-1894, was previously reported as an inhibitor of long chain acyl-CoA synthetase. Pretreatment with triacsin C (500 nM) for 1h enhanced production ofExpand
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Isolation and characterization of fatty acid-binding protein from rat brain.
A fatty acid-binding protein has been identified and isolated from the cytosol fraction of rat brain. The fatty acid-binding protein was purified to homogeneity by gel filtration and preparativeExpand
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