• Publications
  • Influence
Mycolic Acid-Containing Bacteria Induce Natural-Product Biosynthesis in Streptomyces Species
It is shown that mycolic acid-containing bacteria can influence the biosynthesis of cryptic natural products in Streptomyces species, and a new coculture method is developed, called the combined-culture method, which facilitates the screening of natural products. Expand
Cloning of the staurosporine biosynthetic gene cluster from Streptomyces sp. TP-A0274 and its heterologous expression in Streptomyces lividans.
The distribution of the gene for chromopyrrolic acid synthase, the key enzyme for the biosynthesis of indolocarbazole aglycone, in actinomycetes was investigated, and rebD homologs were shown to exist only in the strains producing indolocentrifying antibiotics. Expand
Characterization of the Biosynthetic Gene Cluster of Rebeccamycin from Lechevalieria aerocolonigenes ATCC 39243
The results suggest that indolocarbazole is constructed by oxidative decarboxylation of chromopyrrolic acid generated from two molecules of tryptophan by coupling and that the oxidation state at the C-7 position depends on the additional enzyme(s) encoded by the biosynthetic genes. Expand
Ambuic Acid Inhibits the Biosynthesis of Cyclic Peptide Quormones in Gram-Positive Bacteria
The potential use of ambuic acid as a lead compound of antipathogenic drugs that target the quorum-sensing-mediated virulence expression of gram-positive bacteria is suggested. Expand
Pradimicin A, a Carbohydrate-Binding Nonpeptidic Lead Compound for Treatment of Infections with Viruses with Highly Glycosylated Envelopes, Such as Human Immunodeficiency Virus
Pradimicin A represents the first prototype compound of a nonpeptidic CBA lead and belongs to a conceptually novel type of potential therapeutics for which drug pressure results in the selection of glycan deletions in the HIV gp120 envelope. Expand
Entry of hepatitis C virus and human immunodeficiency virus is selectively inhibited by carbohydrate-binding agents but not by polyanions.
Polyanions such as dextran sulfate-5000 and sulfated polyvinylalcohol inhibited HIV entry but were devoid of any activity against HCV infection, indicating that they act through a different mechanism. Expand
TPU-0031-A and B, new antibiotics of the novobiocin group produced by Streptomyces sp. TP-A0556.
Two novel antibiotics, TPU-0031-A and B, were isolated from the culture broth of an actinomycete strain and showed antibiotic activity against Gram-positive and -negative bacteria. Expand
Characterization and Functional Modification of StaC and RebC, Which Are Involved in the Pyrrole Oxidation of Indolocarbazole Biosynthesis
The diversity of indolocarbazole natural products results from the differences in oxidation states of the pyrroline ring moiety. In the biosynthetic pathways for staurosporine and rebeccamycin, twoExpand
Characterization and carbohydrate specificity of pradimicin S.
Water solubility data reveal the biologically active form of pradimicin S, provide an explanation for previous speculations that PRM-A may contain a second mannose binding site, and expand the understanding of the characteristics that can engender a small molecule with the ability to function as a carbohydrate receptor. Expand