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Development of a multiplex MethyLight assay for the detection of multigene methylation in human colorectal cancer.
In peripheral blood, cell-free methylated DNA has been reported to be a useful biomarker of noninvasive blood screening for the detection of colorectal cancer (CRC), including the genes ALX homeoboxExpand
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Detection of the Most Common G6PD Gene Mutations in Chinese Using Amplification Refractory Mutation System
Glucose-6-phosphate dehydrogenase (G6PD) is the most common human enzymopathy. To date more than 122 mutations in the G6PD gene have been discovered, among which 12 point mutations are found in theExpand
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Immunological characteristics of natural resistance in Microtus fortis to infection with Schistosoma japonicum.
OBJECTIVE To explore the immunological characteristics of natural resistance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area. METHODS Passive transfer ofExpand
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Development and clinical trial of fluorescence PCR diagnostic kit to detect Mycobacterium tuberculosis.
OBJECTIVE To develop a new diagnostic kit for Mycobacterium tuberculosis (TB) detection on the basis of fluorescence PCR (F-PCR) and conduct clinical trial of this kit for assessing its performanceExpand
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Detection of polymerase chain reaction-amplified human immunodeficiency virus type 1 proviral DNA with a digoxigenin-labeled RNA probe and an enzyme-linked immunoassay.
An enzyme-linked immunoassay (EIA) combined with a solution hybridization (SH) reaction was devised to detect human immunodeficiency virus type 1 (HIV-1) provirus amplified by the polymerase chainExpand
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Absence of prolonged immunosilent infection with human immunodeficiency virus in individuals with high-risk behaviors.
PURPOSE The presence in some individuals of a prolonged phase of infection with human immunodeficiency virus type 1 (HIV-1) before seroconversion remains controversial. This study was undertaken toExpand
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Coamplification of HIV type 1 and beta-globin gene DNA sequences in a nonisotopic polymerase chain reaction assay to control for amplification efficiency.
The polymerase chain reaction (PCR) fails to detect HIV-1 sequences in 5% of infected individuals. To screen for false-negative PCR tests, we developed a nonisotopic PCR assay in which sequences fromExpand
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Non-invasive prenatal diagnosis of trisomy 21 by reverse transcriptase multiplex ligation-dependent probe amplification
Abstract Background: Obtaining fetal DNA or RNA by either chorionic villus sampling (CVS) or amniocentesis is currently, the gold standard prenatal diagnosis. However, these invasive procedures carryExpand
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A novel mis-sense mutation (G1381A) in the G6PD gene identified in a Chinese man.
OBJECTIVE To detect new mutations among 29 glucose-6-phosphate dehydrogenase (G6PD) deficient individuals from Yunnan province. METHODS The nitroblue tetrazolium (NBT) method was used to screenExpand
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[Detection of telomerase hTERT gene expression of exfoliated cell in broncho-alveolar lavage fluid].
BACKGROUND & OBJECTIVES The patients with lung carcinoma usually have high telomerase activity, the pseudopositive result is happen frequently when the test is done with traditional method.Expand
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