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A minimal sequence code for switching protein structure and function
We present here a structural and mechanistic description of how a protein changes its fold and function, mutation by mutation. Our approach was to create 2 proteins that (i) are stably folded into 2Expand
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Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency
An efficient Agrobacterium-mediated durum wheat transformation system has been developed for the production of 121 independent transgenic lines. This improved system used Agrobacterium strain AGL1Expand
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Identification and Characterization of microRNAs from Peanut (Arachis hypogaea L.) by High-Throughput Sequencing
Background MicroRNAs (miRNAs) are noncoding RNAs of approximately 21 nt that regulate gene expression in plants post-transcriptionally by endonucleolytic cleavage or translational inhibition. miRNAsExpand
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Mutational tipping points for switching protein folds and functions.
While disordered to ordered rearrangements are relatively common, the ability of proteins to switch from one ordered fold to a completely different fold is generally regarded as rare, and few foldExpand
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The design and characterization of two proteins with 88% sequence identity but different structure and function
To identify a simplified code for conformational switching, we have redesigned two natural proteins to have 88% sequence identity but different tertiary structures: a 3-α helix fold and an α/β fold.Expand
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Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana.
MYB transcription factors (TFs) play pivotal roles in the abiotic stress response in plants, but their characteristics and functions in wheat (Triticum aestivum L.) have not been fully investigated.Expand
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Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR
Quantitative real-time reverse transcription PCR (qRT-PCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. OnlyExpand
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Yeast frataxin solution structure, iron binding, and ferrochelatase interaction.
The mitochondrial protein frataxin is essential for cellular regulation of iron homeostasis. Although the exact function of frataxin is not yet clear, recent reports indicate the protein binds ironExpand
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Genome-Wide Analysis of Fatty Acid Desaturases in Soybean (Glycine max)
Fatty acid desaturases can introduce double bonds into the hydrocarbon chains of fatty acids to produce unsaturated fatty acids. In the present study, 29 full-length desaturase genes were identifiedExpand
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Nurses’ perceptions and practice of physical restraint in China
Background: There is controversy concerning the use of physical restraint. Despite this controversy, some nurses still consider the application of physical restraint unavoidable for some of theirExpand
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