Y. Hayashi

Publications54
h-index20
Citations1,444
Highly Influential Citations39
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Structure-activity relationship of ghrelin: pharmacological study of ghrelin peptides.
Ghrelin, a novel peptide purified from the stomach, is the endogenous ligand of the growth hormone secretagogue receptor. The Ser(3) residue of ghrelin is modified with a lipid n-octanoic acid, a… Expand
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Novel α-Amylase That Is Highly Resistant to Chelating Reagents and Chemical Oxidants from the AlkaliphilicBacillus Isolate KSM-K38
ABSTRACT A novel α-amylase (AmyK38) was found in cultures of an alkaliphilic Bacillus isolate designated KSM-K38. Based on the morphological and physiological characteristics and phylogenetic… Expand
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Membrane protein molecular weight determined by low-angle laser light-scattering photometry coupled with high-performance gel chromatography.
Publisher Summary This chapter presents the basic principle of measurement of the molecular weight of a solubilized membrane protein and describes the practical techniques involved in the… Expand
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Structural similarity of ghrelin derivatives to peptidyl growth hormone secretagogues.
Ghrelin is a 28-amino acid residue endogenous growth hormone secretagogue. Intensive investigations revealed that the N-terminus tetrapeptide, having octanoyl group at Ser(3), is the minimum active… Expand
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Hydrophobic interaction at the subunit interface contributes to the thermostability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus.
We cloned and sequenced the leuB gene encoding 3-isopropylmalate dehydrogenase from Escherichia coli K-12 (JM103). Errors (33 residues) were found and corrected in the sequence previously reported… Expand
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Endosomes differ from plasma membranes in the phospholipid molecular species composition.
125I-Labeled epidermal growth factor was incorporated into and highly concentrated in endosomes of Chinese hamster V79-UF cells during incubation at 37 degrees C for 8 min after binding to its… Expand
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Analysis of acid-base properties of peroxidase and myoglobin.
Two heme-linked groups of horseradish peroxidases, characterized by pKa = 5.8 (isoenzyme A) and 7.3 (isoenzyme C), are believed to be distal amino acid residues and, judging by their acid-base… Expand
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Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant.
An assessment study was carried out to evaluate the performance of the low-angle laser light scattering technique combined with high-performance gel chromatography in the presence of a nonionic… Expand
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The methyl groups in ribosomal RNA from Escherichia coli.
Abstract 1. (1) Escherichia coli cells were labeled with [ Me - 14 C]methionine and [ 3 H]adenosine in the presence or absence of chloramphenicol. The ratio of 14 C to 3 H in RNA was taken as a… Expand
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Transglycosylation reaction of a chitinase purified from Nocardia orientalis.
Chitinase from the culture filtrates of Nocardia orientalis IFO 12806 was purified to apparent homogeneity by precipitation with ammonium sulfate followed by successive chromatography on CM-Sephadex… Expand
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