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Mechanism of Activation of Protein Kinase JAK2 by the Growth Hormone Receptor
The mechanism provides a molecular basis for understanding the oncogenic JAK2 mutations responsible for polycythemia vera and certain other hematologic disorders and may thus be of value in the design of small-molecule inhibitors of clinical applicability. Expand
Lateral mobility of proteins in liquid membranes revisited
It is shown in this work that, for several integral proteins spanning a large range of sizes, the diffusion coefficient is strongly linked to the protein dimensions. Expand
Interplay of α-synuclein binding and conformational switching probed by single-molecule fluorescence
The results imply that the 2 folded structures are preencoded by the α-synuclein amino acid sequence, and are tunable by small-molecule supramolecular states and differing membrane properties, suggesting novel control elements for biological and amyloid regulation of α- Synuclein. Expand
Structural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signaling
It is demonstrated that the MAL interactions defined within the filament represent a template for a conserved mode of TIR-domain interaction involved in both TLR and interleukin-1 receptor signaling. Expand
Quantitative Analysis of Prenylated RhoA Interaction with Its Chaperone, RhoGDI*
It is demonstrated that membrane extraction of Rho GTPase by RhoGDI is a thermodynamically favored passive process that operates through a series of progressively tighter intermediates, much like the one that is mediated by RabGDI. Expand
Single-molecule fluorescence studies of intrinsically disordered proteins.
It is anticipated that SMF and single-molecule methods will find broad application for structural and mechanistic studies of a wide variety of IDPs, both of their disordered conformations, and their ordered ensembles relevant for function and disease. Expand
Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae
A novel single-molecule fluorescence approach is developed to directly observe interactions and stoichiometries in protein complexes from cell extracts and from in vitro synthesized components, revealing an exquisite specificity of interactions between cavin1, cavin2 and cavin3. Expand
Munc18-1 is a molecular chaperone for α-synuclein, controlling its self-replicating aggregation
It is reported that Munc18-1 acts as a chaperone for α-synuclein and controls its aggregative propensity, leading to a neurodegenerative phenotype. Expand
SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species
New functions of the NSP3 and NSP5 proteases of SARS-CoV-2 are discovered, demonstrating that they could directly cleave proteins involved in the host innate immune response, providing an explanatory framework for indepth studies into the pathophysiology of COVID-19. Expand
Microfluidic device for single-molecule experiments with enhanced photostability.
A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking.Expand