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Transformation of intact yeast cells treated with alkali cations.
TLDR
The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
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Transformation of intact yeast cells treated with alkali cations
TLDR
The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
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Molecular insights on DNA delivery into Saccharomyces cerevisiae.
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Degradation of rice bran hemicellulose by Paenibacillus sp. strain HC1: gene cloning, characterization and function of β-D-glucosidase as an enzyme involved in degradation
TLDR
Strain HC1 glycosidases responsible for degradation of rice bran hemicellulose are expected to be useful for structurally determining and molecularly modifying rice brans hemiceLLulose and its derivatives.
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Metabolism of 2‐oxoaldehyde in yeasts
TLDR
An enzyme catalyzing the reduction of methylglyoxal was isolated from Saccharomyces cerevisiae and its enzymatic properties were analyzed and it was tentatively designated the enzyme methyl glyoxal reductase.
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Nucleotide sequence and characterization of a Gene conferring resistance to ethionine in yeast Saccharomyces cerevisiae
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Cloning of the glucokinase gene in Escherichia coli B
TLDR
Some function of Glk may be regulated by a gene(s) near the glk gene, as the introduction of pGK100 into E. coli 112L having a decreased level of GlK activity resulted in the about 15-fold increase in this enzyme activity.
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Paenibacillus sp. strain HC1 xylanases responsible for degradation of rice bran hemicellulose.
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Production of S‐adenosyl‐L‐methionine by Saccharomyces cerevisiae cells carrying a gene for ethionine resistance
A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD‐5D‐H conferred on the yeast cells resistance to seleno‐L‐methionine and capability to produce…
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Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains
TLDR
The gene responsible for the methylglyoxal resistance of Saccharomyces cerevisiae was cloned, and its phenotypic characteristics were investigated and were applicable to the selection of the transformants of wild-type yeast strains having no genetic markers.
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