• Publications
  • Influence
Disposition and Metabolism of a Novel Prostanoid Antiglaucoma Medication, Tafluprost, Following Ocular Administration to Rats
TLDR
The disposition and metabolism of tafluprost, an ester prodrug of the 15,15-difluoro-prostaglandin F2α antiglaucoma agent, have been studied in rats after ocular administration, and radioactivity levels remained greatest in cornea, followed by iris/ciliary body that replaced aqueous humor as the eye tissue containing the second highest radioactivity concentration. Expand
Rapid identification of fatty acids and (O-acyl)-ω-hydroxy fatty acids in human meibum by liquid chromatography/high-resolution mass spectrometry.
TLDR
A rapid liquid chromatography/quadrupole Orbitrap Fourier transform mass spectrometry method for identifying free fatty acids and OAHFAs in human meibum without derivatization shows that retention time correlates with the number of double bonds and carbon chains. Expand
Construction of engineered CHO strains for high-level production of recombinant proteins
TLDR
Using this cell–vector system, engineered CHO strains can reproducibly obtain high producers of recombinant proteins by gene-targeting and gene amplification and in human monoclonal antibody production, the MTX-resistant colonies showed high levels of antibody production. Expand
Drug metabolite heterogeneity in cultured single cells profiled by pico-trapping direct mass spectrometry.
TLDR
Live single-cell MS successfully detected the heterogeneity of drug metabolism in individual living hepatocytes, and showed that tafluprost metabolism varied among individual cells. Expand
Metabolism and ocular tissue distribution of an antiglaucoma prostanoid, tafluprost, after ocular instillation to monkeys.
TLDR
The pharmacokinetic and metabolic properties of this difluorinated prostaglandin in primates are believed to result in clinical benefits of a long-term IOP-lowering effect. Expand
Meibum Color and Free Fatty Acid Composition in Patients With Meibomian Gland Dysfunction.
TLDR
Upper meibum color score was significantly correlated with epiphora and sticky sensation in MGD patients, and this finding may provide insight into the pathogenesis of MGD. Expand
Linoleic acid content of human meibum is associated with telangiectasia and plugging of gland orifices in meibomian gland dysfunction.
TLDR
The relative amount of linoleic acid in meibum was associated with the severity of telangiectasia and plugging of gland orifices in MGD, suggesting that this FFA might contribute to the pathogenesis of these signs. Expand
A label-free impedance-based whole cell assay revealed a new G protein-coupled receptor ligand for mouse microglial cell migration.
TLDR
5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid is a new chemoattractant inducing microglial cell migration through the activation of Gαi, suggesting that PTX-sensitive increase in cellular impedance with the assay is characteristic for ligands of GPCRs involved in microglia cell migration. Expand
Video-microscopy for analysis of molecular dynamics in cells.
TLDR
The results suggest that the present method is useful not only for screening of expectorants but also for the study of molecular mechanisms underlying ciliary beat of tracheal epithelium. Expand
...
1
2
...